DBCO PEG1 NHS Modifier
DBCO-PEG1 NHS ester is a bioorthogonal conjugation reagent designed for the preparation of DBCO-functionalized oligonucleotides from amino-modified DNA, RNA, PNA, aptamers, probes, antisense oligonucleotides, and other synthetic nucleic acids. The NHS ester reacts selectively with primary amines to form a stable amide bond, while the DBCO moiety remains available for strain-promoted azide-alkyne cycloaddition (SPAAC).
Compared with longer PEG spacers such as PEG4 or PEG8, the PEG1 linker provides a shorter and more compact spacer, making it useful when minimal separation between the oligonucleotide and conjugated molecule is desired. Despite its shorter length, the PEG unit improves hydrophilicity compared with direct DBCO attachment and helps reduce steric effects during conjugation.
DBCO-PEG1 reacts rapidly with azide-modified biomolecules under physiological conditions without requiring copper catalysts, making it ideal for sensitive biological applications.
Typical conjugation partners include:
- Azide-modified fluorophores
- Peptides
- Proteins
- Antibodies
- PEG polymers
- Nanoparticles
- Lipids
- Carbohydrates
- Surface coatings
- DNA nanostructures
Typical applications include:
- Copper-free click chemistry (SPAAC)
- Antibody–oligonucleotide conjugates
- Peptide–oligonucleotide conjugates
- Fluorescent labeling
- Nanoparticle functionalization
- Surface immobilization
- Biosensor development
- DNA nanotechnology
- Targeted delivery research
- Custom bioconjugation
Bio-Synthesis offers custom DBCO-PEG1-modified oligonucleotides prepared through post-synthetic labeling of amino-modified oligonucleotides using DBCO-PEG1 NHS ester.
Note: DBCO-PEG1 NHS ester is typically used to modify an existing amino-functionalized oligonucleotide (e.g., Amino C6 or Amino-TEG). It is a post-synthetic conjugation reagent, not a phosphoramidite incorporated directly during automated oligonucleotide synthesis.
Typical Specifications
| Property |
Specification |
| Modification |
DBCO-PEG1 |
| Reagent |
DBCO-PEG1 NHS Ester |
| Reactive Groups |
DBCO (cyclooctyne) and NHS ester |
| Spacer |
PEG1 (single ethylene glycol unit) |
| Spacer Characteristics |
Short, flexible, hydrophilic linker |
| Conjugation Chemistry |
SPAAC (Strain-Promoted Azide-Alkyne Cycloaddition) |
| Copper Requirement |
None (Copper-free) |
| Reaction Partner |
Azide-modified molecules |
| Typical Placement |
5′ end, 3′ end, or internal amino-modified positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, probes |
| Typical Applications |
Copper-free click chemistry, peptide conjugation, antibody conjugation, fluorescent labeling, nanoparticle functionalization, biosensors, targeted delivery |
Product Information
Copper-Free Click Chemistry
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Agard NJ, Prescher JA, Bertozzi CR. A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. J Am Chem Soc. 2004;126(46):15046-15047.
- Jewett JC, Bertozzi CR. Cu-free click cycloaddition reactions in chemical biology. Chem Soc Rev. 2010;39:1272-1279.
- Sletten EM, Bertozzi CR. Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed. 2009;48:6974-6998.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
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