DBCO PEG5 NHS Modifier
DBCO-PEG5 NHS ester is a bioorthogonal conjugation reagent used to prepare DBCO-functionalized oligonucleotides by reacting with amino-modified DNA, RNA, PNA, aptamers, probes, antisense oligonucleotides, siRNA, and other synthetic nucleic acids. The NHS ester rapidly forms stable amide bonds with primary amines, while the DBCO moiety remains available for strain-promoted azide-alkyne cycloaddition (SPAAC).
The PEG5 spacer offers an intermediate linker length that improves aqueous solubility and molecular flexibility while maintaining a relatively compact structure. Compared with shorter PEG linkers, PEG5 provides increased separation between the oligonucleotide and the attached biomolecule, helping reduce steric hindrance and improving click conjugation efficiency.
DBCO-PEG5 reacts specifically with azide-modified molecules under mild physiological conditions without requiring copper catalysts, making it ideal for applications involving sensitive nucleic acids, proteins, antibodies, nanoparticles, and living cells.
Typical conjugation partners include:
- Azide-modified fluorophores
- Peptides
- Proteins
- Antibodies
- PEG polymers
- Nanoparticles
- Lipids
- Carbohydrates
- Surface coatings
- DNA nanostructures
Typical applications include:
- Copper-free click chemistry (SPAAC)
- Antibody–oligonucleotide conjugates
- Peptide–oligonucleotide conjugates
- Fluorescent labeling
- Protein labeling
- Nanoparticle functionalization
- Surface immobilization
- Biosensor development
- DNA nanotechnology
- Targeted drug delivery
- Molecular imaging
Bio-Synthesis offers custom DBCO-PEG5-modified oligonucleotides prepared by post-synthetic conjugation of amino-modified oligonucleotides using DBCO-PEG5 NHS ester for highly efficient bioorthogonal coupling.
Note: Like other DBCO-PEG NHS esters, DBCO-PEG5 NHS ester is a post-synthetic labeling reagent used to react with amino-modified oligonucleotides (e.g., Amino C6 or Amino-TEG). The resulting DBCO-PEG5-modified oligonucleotide is then ready for efficient SPAAC copper-free click conjugation with azide-functionalized molecules.
Typical Specifications
| Property |
Specification |
| Modification |
DBCO-PEG5 |
| Reagent |
DBCO-PEG5 NHS Ester |
| Reactive Groups |
DBCO (cyclooctyne) and NHS ester |
| Spacer |
PEG5 (pentaethylene glycol) |
| Spacer Characteristics |
Flexible, hydrophilic, low steric hindrance |
| Conjugation Chemistry |
SPAAC (Strain-Promoted Azide-Alkyne Cycloaddition) |
| Copper Requirement |
None (Copper-free) |
| Reaction Partner |
Azide-modified molecules |
| Typical Placement |
5′ end, 3′ end, or internal amino-modified positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, probes |
| Typical Applications |
Copper-free click chemistry, antibody conjugation, peptide conjugation, fluorescent labeling, nanoparticle functionalization, biosensors, targeted delivery, molecular imaging |
Product Information
Copper-Free Click Chemistry
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Agard NJ, Prescher JA, Bertozzi CR. A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. J Am Chem Soc. 2004;126(46):15046-15047.
- Jewett JC, Bertozzi CR. Cu-free click cycloaddition reactions in chemical biology. Chem Soc Rev. 2010;39:1272-1279.
- Sletten EM, Bertozzi CR. Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed. 2009;48:6974-6998.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
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