Dialkylaminocoumarin (DEAC)
Diethylaminocoumarin (DEAC) is a member of the coumarin family of fluorescent dyes and is widely used as a compact fluorescent label for nucleic acids, proteins, and other biomolecules. The dye is characterized by strong absorption in the near-UV region and bright blue fluorescence emission, making it an excellent choice for applications requiring short-wavelength excitation.
When incorporated into DNA, RNA, PNA, aptamers, or oligonucleotide probes, DEAC enables sensitive fluorescence detection while introducing minimal steric interference due to its relatively small molecular size. The fluorophore is frequently used as a FRET donor, in fluorescence polarization assays, biosensor development, hybridization studies, and molecular imaging.
Bio-Synthesis offers custom DEAC (DECA)-modified oligonucleotides with labeling at the 5′ end, 3′ end, or internal positions, depending on the synthesis strategy and project requirements.
Typical applications include:
- Fluorescence spectroscopy
- Fluorescence microscopy
- FRET donor labeling
- Hybridization assays
- Molecular beacons
- Biosensor development
- Aptamer characterization
- Protein–DNA interaction studies
- Protein–RNA interaction studies
- DNA nanotechnology
Typical Specifications
| Property |
Specification |
| Modification |
Diethylaminocoumarin (DEAC/DECA) |
| Dye Family |
Coumarin fluorophore |
| Excitation Maximum |
Approximately 430–435 nm* |
| Emission Maximum |
Approximately 470–480 nm* |
| Fluorescence Color |
Blue |
| Typical Placement |
5′ end, 3′ end, or internal positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, aptamers, molecular probes |
| Typical Applications |
Fluorescence microscopy, FRET donor labeling, biosensors, hybridization assays, molecular imaging |
*Optical properties may vary slightly depending on the specific DEAC derivative or linker chemistry.
Product Information
Dialkylaminocoumarin (DEAC)
7-Diethylaminocoumarin (DEAC)
Fluorescent Dye Modifications
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Haugland RP. The Handbook: A Guide to Fluorescent Probes and Labeling Technologies. Molecular Probes (Thermo Fisher Scientific).
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Valeur B, Berberan-Santos MN. Molecular Fluorescence: Principles and Applications. 2nd Edition. Wiley-VCH; 2012.
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