DNP-TEG (2,4-Dinitrophenyl)
DNP-TEG (2,4-dinitrophenyl-triethylene glycol) is an affinity-labeling modification that introduces the well-characterized 2,4-dinitrophenyl (DNP) hapten onto an oligonucleotide through a flexible triethylene glycol (TEG) spacer. The TEG linker increases hydrophilicity and distances the DNP group from the nucleic acid, improving accessibility for antibody recognition while minimizing steric hindrance.
DNP is one of the most extensively studied haptens in immunochemistry. Oligonucleotides labeled with DNP-TEG can be recognized by anti-DNP antibodies, enabling highly specific detection, capture, immobilization, and analytical workflows. Because the interaction is based on antibody recognition rather than nucleic acid hybridization, DNP labeling provides a versatile affinity tag for numerous molecular biology and diagnostic applications.
Bio-Synthesis offers custom DNP-TEG-modified oligonucleotides for DNA, RNA, PNA, aptamers, probes, antisense oligonucleotides, and other synthetic nucleic acids. The modification may be incorporated at the 5′ end, 3′ end, or internal positions, depending on the synthesis strategy and project requirements.
Typical applications include:
- Anti-DNP antibody detection
- Immunoassays
- ELISA-based assays
- Affinity capture
- Biosensor development
- Surface immobilization
- Molecular recognition studies
- Flow cytometry reagents
- Aptamer assays
- Diagnostic probe development
Typical Specifications
| Property |
Specification |
| Modification |
DNP-TEG |
| Full Name |
2,4-Dinitrophenyl-Triethylene Glycol |
| Modification Type |
Hapten affinity label |
| Affinity Target |
Anti-DNP antibodies |
| Spacer |
Triethylene glycol (TEG) |
| Spacer Characteristics |
Flexible, hydrophilic, reduced steric hindrance |
| Typical Placement |
5′ end, 3′ end, or internal positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, aptamers, molecular probes |
| Typical Applications |
Immunoassays, ELISA, affinity capture, antibody detection, biosensors, diagnostic assays |
Product Information
DNP-TEG (2,4-Dinitrophenyl)
2,4-dinitrophenyl-triethylene glycol
Affinity Tags, Hapten Labels
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Green NM. Avidin and streptavidin. Methods in Enzymology. 1990;184:51-67.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Harlow E, Lane D. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press; 1988.
- Goodchild J. Oligonucleotide conjugates and their applications. Bioconjugate Chemistry. 1990;1:165-187.
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