Dyomic DY-647, Far Red Emission
DY-647® is a bright far-red fluorescent dye developed by Dyomics GmbH for fluorescence imaging, flow cytometry, confocal microscopy, and molecular diagnostics. It exhibits strong absorption around 654 nm with peak fluorescence emission at approximately 672 nm, making it well suited for excitation using standard 633 nm or 640 nm laser systems.
DY-647 provides high fluorescence intensity, excellent photostability, and low background autofluorescence compared with shorter wavelength fluorophores. These characteristics make it an excellent choice for dual-labeled probes, FISH probes, molecular beacons, qPCR assays, oligonucleotide conjugates, protein labeling, and super-resolution microscopy.
For oligonucleotide synthesis, DY-647 is commonly introduced through 5′ modification, 3′ modification, or internal amino-modified nucleotides, allowing flexible placement within DNA, RNA, siRNA, ASOs, aptamers, and other custom oligonucleotide constructs.
Because of its spectral properties, DY-647 is frequently selected as a functional alternative to Alexa Fluor® 647, ATTO® 647N, and CF® 647 in multicolor fluorescence experiments.
Typical Specifications & Technical Notes
| Property |
Specification |
| Dye Name |
DY-647® |
| Manufacturer |
Dyomics GmbH |
| Dye Family |
Red Excitation Fluorescent Dye |
| Excitation Maximum |
654 nm |
| Emission Maximum |
672 nm |
| Recommended Laser |
633 nm, 640 nm |
| Emission Color |
Far Red |
| Photostability |
Excellent |
| Brightness |
High |
| Water Solubility |
High |
| Available Labeling Positions |
5′, 3′, Internal |
| Compatible Oligonucleotides |
DNA, RNA, siRNA, ASO, Aptamers, Molecular Beacons, FISH Probes |
| Typical Equivalent |
Alexa Fluor® 647 • ATTO® 647N • CF® 647 |
| Typical Applications |
FISH, flow cytometry, confocal microscopy, super-resolution imaging, qPCR probes, molecular beacons, RNA imaging, aptamer labeling |
| Technical Notes |
• Optimized for 633 nm and 640 nm laser excitation.
• Excellent photostability with high fluorescence intensity.
• Low cellular autofluorescence due to far-red emission.
• Compatible with 5′, 3′ and internal oligonucleotide labeling.
• Commonly paired with DYQ-660, DYQ-661, BHQ-3, BBQ-650 and other far-red quenchers. |
Product Information
Dyomic DY-647, Far Red Emission
dual HPLC/SEC is required
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Dyomics GmbH. DY-647 Fluorescent Dye Technical Information and Spectral Data. Dyomics GmbH, Jena, Germany. Available at: https://dyomics.com
- Bio-Synthesis Inc. Dyomics Fluorescent Dye Modification Listing. Available at: https://www.biosyn.com/oligonucleotideproduct/dyomics-fluorescent-dye-modification-listing.aspx
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155.
- Haugland RP. The Handbook: A Guide to Fluorescent Probes and Labeling Technologies. Molecular Probes (Thermo Fisher Scientific).
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