Dyomic Dy-350XL UV-MegaStokes
DY-350XL UV MegaStokes® Fluorescent Dye Oligonucleotide Modification
DY-350XL UV MegaStokes® is an advanced fluorescent dye developed by Dyomics GmbH as part of its UV MegaStokes® fluorophore family. Unlike conventional fluorophores that exhibit relatively small differences between excitation and emission wavelengths, UV MegaStokes® dyes are specifically engineered with an exceptionally large Stokes shift, allowing efficient excitation with ultraviolet light while emitting fluorescence at significantly longer wavelengths.
The large spectral separation dramatically reduces excitation light bleed-through into the emission channel, minimizing background fluorescence and improving signal-to-noise ratios. These properties simplify optical filter selection and make DY-350XL UV MegaStokes® particularly valuable for high-sensitivity fluorescence detection, quantitative imaging, and multiplex fluorescence assays.
Bio-Synthesis offers custom DY-350XL UV MegaStokes®-modified oligonucleotides for DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, PCR probes, molecular beacons, FISH probes, CRISPR guide RNAs, and numerous custom nucleic acid constructs. The dye may be incorporated at the 5′ terminus, 3′ terminus, or internally through amino-modified nucleotides using appropriate linker chemistries.
DY-350XL UV MegaStokes® is fully compatible with many Bio-Synthesis oligonucleotide modifications, including:
- Biotin
- Digoxigenin
- Amino modifiers
- Thiol modifiers
- PEG linkers
- Azide
- DBCO
- BCN
- Tetrazine
- GalNAc
- Cholesterol
- Peptide conjugates
- Lipid conjugates
- Fluorescent quenchers
The dye incorporates Dyomics' hydrophilic molecular design to improve aqueous solubility while minimizing nonspecific adsorption to biological samples and laboratory surfaces. Combined with excellent photostability, DY-350XL UV MegaStokes® supports prolonged fluorescence imaging and repeated excitation without significant photobleaching, making it well suited for demanding fluorescence microscopy and analytical applications.
Typical research applications include:
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Fluorescence in situ hybridization (FISH)
- Multiplex fluorescence imaging
- Quantitative PCR probes
- Molecular beacon assays
- Hybridization probes
- DNA and RNA labeling
- Biosensor development
- DNA nanotechnology
- High-sensitivity fluorescence detection
Each DY-350XL UV MegaStokes®-modified oligonucleotide is synthesized using automated solid-phase phosphoramidite chemistry and is available with optional HPLC or PAGE purification. Comprehensive quality control includes HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence verification to confirm successful dye incorporation and product purity.
| Property |
Specification |
| Modification |
DY-350XL UV MegaStokes® |
| Modification Class |
Large Stokes Shift Fluorescent Dye |
| Dye Family |
Dyomics® UV MegaStokes® Series |
| Excitation Source |
Ultraviolet (UV) |
| Emission |
Blue to blue-green (derivative dependent) |
| Stokes Shift |
Very Large (MegaStokes®) |
| Photostability |
Excellent |
| Water Solubility |
High |
| Typical Attachment |
5′ end, 3′ end, or internal amino linker |
| Compatible Molecules |
DNA, RNA, PNA, LNA, siRNA, ASOs, Aptamers |
| Primary Applications |
Microscopy, FISH, flow cytometry, multiplex fluorescence, biosensors, PCR probes |
Exact excitation/emission maxima depend on the specific reactive derivative (NHS ester, maleimide, phosphoramidite, etc.) and should be confirmed using the current Dyomics® technical datasheet.
Research Use Only (RUO): Unless otherwise specified, DY-350XL UV MegaStokes®-modified oligonucleotides are supplied exclusively for research applications.
Trademark Notice: DY®, Dyomics®, and MegaStokes® are trademarks of Dyomics GmbH. Bio-Synthesis provides custom oligonucleotide synthesis and conjugation services using customer-specified or commercially available DY® dyes and is not affiliated with or endorsed by Dyomics GmbH.
Product Information
Dyomic Dy-350XL UV-MegaStokes
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Dyomics GmbH. DY® Fluorophores and UV MegaStokes® Product Guide. Dyomics GmbH, Jena, Germany.
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T. Quantum dots versus organic dyes as fluorescent labels. Nature Methods. 2008;5(9):763-775.
- Haugland RP. Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes (Thermo Fisher Scientific).
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