Dyomic DY-405, Violet Emission
DY-405 Fluorescent Dye Oligonucleotide Modification
DY-405 is a high-performance fluorescent dye developed by Dyomics GmbH for advanced biomolecular labeling applications. Designed for excitation with common 405 nm violet lasers, the dye delivers bright blue fluorescence while maintaining excellent photostability and high quantum efficiency. These characteristics make DY-405 particularly useful on modern fluorescence microscopes, confocal imaging systems, and flow cytometers equipped with standard 405 nm laser sources.
Compared with traditional UV-excited fluorophores, DY-405 offers improved compatibility with widely available violet laser instrumentation while maintaining strong fluorescence intensity. Its hydrophilic molecular design reduces nonspecific interactions and improves aqueous solubility, resulting in reproducible labeling and high-quality fluorescence signals.
Bio-Synthesis offers custom DY-405-modified oligonucleotides for DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, PCR probes, hybridization probes, molecular beacons, FISH probes, CRISPR guide RNAs, and numerous other synthetic nucleic acid constructs. DY-405 can be incorporated at the 5′ terminus, 3′ terminus, or internally through amino-modified nucleotides using appropriate linker chemistries.
DY-405 is compatible with numerous additional Bio-Synthesis modifications including:
- Biotin
- Digoxigenin
- Amino modifiers
- Thiol modifiers
- PEG linkers
- Azide
- DBCO
- BCN
- Tetrazine
- Cholesterol
- GalNAc
- Peptide conjugates
- Lipid conjugates
- Fluorescent quenchers
Because DY-405 is excited by the widely available 405 nm laser, it is particularly valuable for multiplex fluorescence experiments together with green, yellow, orange, red, and near-infrared fluorophores. The excellent photostability supports repeated imaging and prolonged exposure with minimal photobleaching.
Typical research applications include:
- DNA and RNA fluorescent labeling
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Fluorescence in situ hybridization (FISH)
- Molecular beacon probes
- Hybridization assays
- qPCR probe development
- Aptamer labeling
- DNA nanotechnology
- Multiplex fluorescence imaging
- Biosensor development
Each DY-405-modified oligonucleotide is synthesized using automated solid-phase phosphoramidite chemistry and is available with optional HPLC or PAGE purification. Quality control includes HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence verification to confirm successful dye incorporation and product purity.
| Property |
Specification |
| Modification |
DY-405 Fluorescent Dye |
| Modification Class |
Fluorescent Dye |
| Dye Family |
Dyomics® DY Series |
| Fluorescence Color |
Blue |
| Typical Excitation Maximum |
405 nm* |
| Typical Emission Maximum |
Approximately 450–470 nm* |
| Excitation Source |
405 nm violet laser |
| Photostability |
Excellent |
| Water Solubility |
High |
| Typical Attachment |
5′ end, 3′ end, or internal amino linker |
| Compatible Molecules |
DNA, RNA, PNA, LNA, siRNA, ASOs, Aptamers |
| Primary Applications |
Microscopy, flow cytometry, FISH, PCR probes, multiplex fluorescence imaging |
*Spectral properties may vary slightly depending on the reactive derivative (phosphoramidite, NHS ester, maleimide, etc.) and manufacturer specifications.
Research Use Only (RUO): Unless otherwise specified, DY-405-modified oligonucleotides are supplied exclusively for research applications.
Trademark Notice: DY® and Dyomics® are trademarks of Dyomics GmbH. Bio-Synthesis provides custom oligonucleotide synthesis and conjugation services using customer-specified or commercially available DY® dyes and is not affiliated with or endorsed by Dyomics GmbH.
Product Information
Dyomic DY-405, Violet Emission
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Dyomics GmbH. DY® Fluorophores Product Catalog and Spectral Properties. Dyomics GmbH, Jena, Germany.
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T. Quantum dots versus organic dyes as fluorescent labels. Nature Methods. 2008;5(9):763-775.
- Haugland RP. Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes (Thermo Fisher Scientific).
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