Dyomic DY-431, Green Emission
DY-431 is a high-performance fluorescent dye developed by Dyomics GmbH for advanced fluorescence labeling applications. As a member of the Dyomics® DY Series, DY-431 combines high fluorescence intensity, excellent photostability, and superior aqueous solubility to provide reliable labeling of oligonucleotides and other biomolecules for research and diagnostic assay development.
Designed for excitation by violet laser sources, DY-431 produces bright blue fluorescence with excellent resistance to photobleaching. Its hydrophilic molecular design minimizes nonspecific adsorption while improving labeling efficiency and fluorescence reproducibility. These characteristics make DY-431 particularly useful for high-sensitivity fluorescence microscopy, confocal imaging, flow cytometry, fluorescence in situ hybridization (FISH), and multiplex detection systems.
Bio-Synthesis offers custom DY-431-modified oligonucleotides for a broad range of nucleic acid formats, including:
- DNA oligonucleotides
- RNA oligonucleotides
- siRNA
- Antisense oligonucleotides (ASOs)
- PNA
- LNA/BNA oligonucleotides
- Aptamers
- PCR probes
- Hybridization probes
- Molecular Beacon probes
- FISH probes
- CRISPR guide RNAs
DY-431 may be incorporated at the 5′ terminus, 3′ terminus, or internally through amino-modified nucleotides using suitable linker chemistries.
The dye is compatible with numerous additional Bio-Synthesis modifications, including:
- Biotin
- Digoxigenin
- Amino modifiers
- Thiol modifiers
- PEG linkers
- Azide
- DBCO
- BCN
- Tetrazine
- Cholesterol
- GalNAc
- Peptide conjugates
- Lipid conjugates
- Fluorescent quenchers
Its spectral properties allow DY-431 to function effectively in multiplex fluorescence experiments together with green-, yellow-, orange-, red-, and near-infrared fluorophores. The dye's excellent photostability enables prolonged imaging sessions while maintaining consistent fluorescence intensity.
Typical research applications include:
- DNA and RNA fluorescent labeling
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Fluorescence in situ hybridization (FISH)
- Multiplex fluorescence imaging
- Quantitative PCR probes
- Molecular Beacon assays
- Hybridization assays
- Aptamer imaging
- DNA nanotechnology
- Biosensor development
Every DY-431-modified oligonucleotide is synthesized using automated solid-phase phosphoramidite chemistry and is available with optional HPLC or PAGE purification. Comprehensive analytical quality control includes HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence verification to confirm sequence integrity, purity, and successful dye incorporation.
| Property |
Specification |
| Modification |
DY-431 Fluorescent Dye |
| Modification Class |
Fluorescent Dye |
| Dye Family |
Dyomics® DY Series |
| Fluorescence Color |
Blue |
| Excitation Source |
Violet laser (approximately 405–430 nm)* |
| Emission |
Blue region* |
| Photostability |
Excellent |
| Water Solubility |
High |
| Typical Attachment |
5′ end, 3′ end, or internal amino linker |
| Compatible Molecules |
DNA, RNA, PNA, LNA, siRNA, ASOs, Aptamers |
| Primary Applications |
Microscopy, flow cytometry, FISH, PCR probes, multiplex fluorescence imaging |
*Exact excitation/emission maxima depend on the specific reactive derivative (NHS ester, maleimide, phosphoramidite, etc.) and should be confirmed using the current Dyomics® technical datasheet.
Research Use Only (RUO): Unless otherwise specified, DY-431-modified oligonucleotides are supplied exclusively for research applications.
Trademark Notice: DY® and Dyomics® are trademarks of Dyomics GmbH. Bio-Synthesis provides custom oligonucleotide synthesis and conjugation services using customer-specified or commercially available DY® dyes and is not affiliated with or endorsed by Dyomics GmbH.
Product Information
Dyomic DY-431, Green Emission
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Dyomics GmbH. DY® Fluorophores Product Guide and Spectral Properties. Dyomics GmbH, Jena, Germany.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Haugland RP. Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes (Thermo Fisher Scientific).
- Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T. Quantum dots versus organic dyes as fluorescent labels. Nature Methods. 2008;5(9):763-775.
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