Fluorescein Dye Listing
Fluorescein dyes are among the most widely used fluorescent labels for DNA, RNA, PNA, peptides, proteins, and antibodies. Based on the xanthene fluorophore scaffold, fluorescein derivatives provide bright green to yellow-green fluorescence, high quantum yields, and excellent compatibility with common laser and filter sets. Popular derivatives such as FAM™, FITC, HEX™, TET™, JOE™, and Oregon Green® are widely used for qPCR probes, DNA sequencing, FISH, molecular diagnostics, fluorescence microscopy, and flow cytometry. Bio-Synthesis offers custom oligonucleotide synthesis with a broad selection of fluorescein-based dye modifications for research and diagnostic applications.
| Fluorescein Dye |
Ex (nm) |
Em (nm) |
Recommended Laser |
Emission Color |
Typical Applications |
| Fluorescein |
494 |
521 |
488 nm |
Green |
General fluorescence labeling |
| FITC (Fluorescein Isothiocyanate) |
495 |
519 |
488 nm |
Green |
Protein, antibody and peptide labeling |
| FAM™ (6-FAM) |
495 |
520 |
488 nm |
Green |
qPCR probes, DNA sequencing, oligonucleotide labeling |
| TET™ |
521 |
536 |
514/520 nm |
Yellow-Green |
Multiplex PCR |
| HEX™ |
535 |
556 |
532/543 nm |
Yellow-Green |
qPCR, molecular diagnostics |
| JOE™ |
520 |
548 |
514/520 nm |
Yellow |
DNA sequencing, PCR probes |
| Oregon Green® 488 |
496 |
524 |
488 nm |
Green |
Live-cell imaging |
| Oregon Green® 514 |
511 |
530 |
514 nm |
Green |
Confocal microscopy |
| Eosin |
518 |
545 |
514/520 nm |
Yellow-Green |
Photochemistry, fluorescence labeling |
| Erythrosin |
530 |
550 |
532 nm |
Yellow |
Specialized fluorescence applications |
Trademark Notice: FAM™, FITC, Oregon Green®, HEX™, TET™, and JOE™ are trademarks or registered trademarks of their respective owners. Bio-Synthesis provides custom oligonucleotide labeling with compatible fluorescein-based dyes and is not affiliated with or endorsed by the respective trademark holders.
Product Information
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
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