Non-Cleavable Crosslinkers for Oligonucleotide Conjugation

Bio-Synthesis offers a diverse range of non-cleavable crosslinkers for stable and permanent oligonucleotide conjugation. These linkers are ideal when a long-lasting, irreversible bond is required between oligonucleotides and other biomolecules such as peptides, proteins, antibodies, or nanoparticles.

What Are Non-Cleavable Crosslinkers?

Non-cleavable crosslinkers are chemical linkers that form stable covalent bonds between an oligonucleotide and a target molecule. Unlike cleavable linkers, they remain intact under biological conditions and do not break down in the presence of reducing agents, enzymes, acidic pH, or other intracellular triggers.

This makes them ideal for applications requiring durable labeling, tracking, or immobilization—especially in diagnostics, surface attachment, and in vitro assays.

Non-Cleavable Linkers We Offer

Linker Type	Functional Group Example	Reactivity	Applications
SMCC	NHS-maleimide	Amine to thiol	Antibody-oligo conjugation
Sufo-SMCC	Water-solubable NHS-maleimide	Amine to thiol	Aqueous conjguation workflows
MBS	NHS ester-maleimide	Amine to thiol	Bioconjugation of proteins and oligos
EMCS	NHS-maleimide spacer	Long flexible spacer	Distance-sensitive biomoleccule conjugations
BM(PEG)n	PEG-maleimide NHS esters	Amine to thiol	Improves solubility and reduces aggregation
DSS	NHS-NHS homobiofunctional	Amine to amine	Protein-protein or protein-oligo coupling
EDC	Zero-length carboxy Linkers	Carobxyl to amide	Label-free coupling of oligos to peptides

Need a specific linker not listed above? Contact us for custom synthesis options.

Applications

• Stable bioconjugates for microarrays, biosensors, and diagnostic chips
• Surface immobilization of oligonucleotides on beads, glass, or nanoparticles
• Protein-oligo conjugates for ELISA or lateral flow assays
• Permanent fluorescent labeling or drug-oligo conjugation

Why Choose Bio-Synthesis?

• 45+ years of custom synthesis experience
• Full range of linkers: cleavable, non-cleavable, and stimuli-responsive
• Expertise in oligo–protein, oligo–peptide, and oligo–small molecule conjugates
• Custom design, optimization, and scale-up services

Requst a Quote for Custom Conjugation

Our team can help you select the most suitable non-cleavable crosslinker for your specific oligonucleotide and application. We offer custom synthesis, scale-up production, and analytical validation to meet your project requirements. Request a Custom Quote or Contact Our Scientists