Bio-Synthesis offers a diverse range of non-cleavable crosslinkers for stable and permanent oligonucleotide conjugation. These linkers are ideal when a long-lasting, irreversible bond is required between oligonucleotides and other biomolecules such as peptides, proteins, antibodies, or nanoparticles.
What Are Non-Cleavable Crosslinkers?
Non-cleavable crosslinkers are chemical linkers that form stable covalent bonds between an oligonucleotide and a target molecule. Unlike cleavable linkers, they remain intact under biological conditions and do not break down in the presence of reducing agents, enzymes, acidic pH, or other intracellular triggers.
This makes them ideal for applications requiring durable labeling, tracking, or immobilization—especially in diagnostics, surface attachment, and in vitro assays.
Non-Cleavable Linkers We Offer
| Linker Type |
Functional Group Example |
Reactivity |
Applications |
| SMCC |
NHS-maleimide |
Amine to thiol |
Antibody-oligo conjugation |
| Sufo-SMCC |
Water-solubable NHS-maleimide |
Amine to thiol |
Aqueous conjguation workflows |
| MBS |
NHS ester-maleimide |
Amine to thiol |
Bioconjugation of proteins and oligos |
| EMCS |
NHS-maleimide spacer |
Long flexible spacer |
Distance-sensitive biomoleccule conjugations |
| BM(PEG)n |
PEG-maleimide NHS esters |
Amine to thiol |
Improves solubility and reduces aggregation |
| DSS |
NHS-NHS homobiofunctional |
Amine to amine |
Protein-protein or protein-oligo coupling |
| EDC |
Zero-length carboxy Linkers |
Carobxyl to amide |
Label-free coupling of oligos to peptides |
Need a specific linker not listed above? Contact us for custom synthesis options.
Applications
- Stable bioconjugates for microarrays, biosensors, and diagnostic chips
- Surface immobilization of oligonucleotides on beads, glass, or nanoparticles
- Protein-oligo conjugates for ELISA or lateral flow assays
- Permanent fluorescent labeling or drug-oligo conjugation
Why Choose Bio-Synthesis?
- 45+ years of custom synthesis experience
- Full range of linkers: cleavable, non-cleavable, and stimuli-responsive
- Expertise in oligo–protein, oligo–peptide, and oligo–small molecule conjugates
- Custom design, optimization, and scale-up services
Requst a Quote for Custom Conjugation
Our team can help you select the most suitable non-cleavable crosslinker for your specific oligonucleotide and application. We offer custom synthesis, scale-up production, and analytical validation to meet your project requirements. Request a Custom Quote or Contact Our Scientists
Product Information
Non-Cleavable Crosslinkers for Oligonucleotide Conjugation
Noncleavable Crosslinkers
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
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