Cy5.5 Fluor Dye, NIR Emission

Cy5.5 Fluor Dye NHS Ester is a reactive near-infrared cyanine dye that selectively labels primary amines through its N-hydroxysuccinimide (NHS) ester group. Following conjugation to amino-modified DNA, RNA, PNA, aptamers, antisense oligonucleotides, siRNA, or molecular probes, the resulting fluorescent oligonucleotide exhibits bright near-infrared emission with excellent sensitivity and low background fluorescence.

Compared with Cy5, Cy5.5 emits at longer wavelengths, reducing interference from biological autofluorescence while providing deeper optical penetration in biological samples. These properties make Cy5.5 a popular fluorophore for in vivo imaging, fluorescence-guided studies, multiplex assays, and high-sensitivity analytical applications.

Bio-Synthesis offers custom Cy5.5-labeled oligonucleotides prepared by reacting Cy5.5 NHS ester with amino-modified oligonucleotides. Labeling can be performed at the 5′ end, 3′ end, or internal amino-modified positions, depending on the experimental design.

Typical applications include:

• Near-infrared fluorescence imaging
• In vivo imaging
• Flow cytometry
• Fluorescence microscopy
• Confocal microscopy
• FRET acceptor labeling
• Hybridization probes
• Molecular beacons
• Biosensor development
• DNA nanotechnology
• Multiplex fluorescence assays

Typical Specifications

Product Information

 

Product Name:

Cy5.5 Fluor Dye, NIR Emission

Category:

Near infrared fluorescent dye

Modification Code:

[CY5.5-NHS]

Chemical Formula:

C39H42N2O4P

Exact Mass:

633.29

Formula Weight:

633.76

Excitation (nm):

675

            

Emission (nm):

674

            

Extinction Coefficient, ε(λ):

209,000

Structure:

Purification:

Dual HPLC/SEC required

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. Mujumdar RB, Ernst LA, Mujumdar SR, Lewis CJ, Waggoner AS. Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconjugate Chemistry. 1993;4(2):105-111.
2. Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
3. Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
4. Waggoner A. Fluorescent labels for proteomics and genomics. Current Opinion in Chemical Biology. 2006;10:62-66.

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