DEACM Photo Caged-dG Oligo Modification

DEACM Caged-dG is a photoactivatable guanosine modification designed for controlling nucleic acid function with light. The guanine base is masked with the 7-diethylaminocoumarin-4-ylmethyl (DEACM) photolabile protecting group, preventing normal hydrogen bonding and disrupting hybridization or enzymatic recognition until photolysis occurs.

Unlike traditional o-nitrobenzyl photocages that require shorter UV wavelengths, DEACM can typically be removed using approximately 405 nm violet light, reducing phototoxicity and making it more compatible with live-cell imaging and biological experiments.

Following irradiation, the DEACM group is rapidly cleaved, regenerating native deoxyguanosine (dG) and restoring normal DNA hybridization and biological activity. This enables researchers to activate oligonucleotide function with precise spatial and temporal control.

Bio-Synthesis offers custom DEACM Caged-dG-modified oligonucleotides for:

• Light-activated PCR
• Photoactivatable CRISPR systems
• Optogenetics
• DNA nanotechnology
• Controlled hybridization
• Gene regulation
• Molecular switches
• Synthetic biology
• DNA computing
• Time-resolved biological studies

Specifications & Technical Notes

Product Information

 

Product Name:

DEACM Photo Caged-dG Oligo Modification

Category:

photocleavable, Light-Activated

Modification Code:

[DEACM-dG]

Structure:

Purification:

dual HPLC/SEC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.

Related Products:

NPOM-caged dT

References/Citations:

1. Hagen V, et al. Coumarinylmethyl esters for efficient photorelease using visible light. Angewandte Chemie International Edition. 2005.
2. Mayer G, Heckel A. Biologically active molecules with a "light switch". Angewandte Chemie International Edition. 2006;45:4900-4921.
3. Deiters A. Light activation as a method of regulating and studying gene expression. Current Opinion in Chemical Biology. 2009;13:678-686.
4. Young DD, Deiters A. Photochemical control of biological processes. Organic & Biomolecular Chemistry. 2007;5:999-1005.

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