Oligonucleotide Synthesis

Guaranteed Quality

At Bio-Synthesis, we pride ourselves on our reputation for high quality nucleic acid synthesis.

As part of our long-standing commitment to quality, we employ the following standards for quality assurance:

• All nucleic acid synthesis products (including RNA, siRNAs, DNA, PNA) are analyzed by mass spectrometry and quantified at least twice by uv spectrophotometry (unless no post-synthesis processing is requested)
• MALDI-TOFMS for all standard DNA, RNA, siRNA and PNA synthesis
• HPLC, PAGE, RNase Free HPLC and PAGE for purification of DNA and RNA products
• UPLC or CGE for quality analysis when appropriate
• Custom QC methods and documentation are available upon request

Quality control documentation available upon request includes:

• Mass Spectrometry traces
• UPLC or CGE analysis
• Duplex or individual strand purity analysis
• Certificate of Analysis
• Endotoxin certification
• Custom QC documentation

For all mid-scale synthesis projects (200 mg - 10 g), we adhere to a stringent manufacturing process:

• HPLC purification highly recommended to achieve optimal purity
• Tangiential Flow Filtration (TFF) utilized for premium salt removal
• Batch records maintained on all synthesis projects (we follow GMP guidelines, but are not GMP certified)
• Endotoxin testing included on all mid-scale orders
• Data deliverables include MS, UPLC, endotoxin results, and a certificate of analysis

If you are dissatisfied and report an oligo problem within 30 days of order receipt, we will gladly remake your oligo free of charge or provide a credit for the purchase amount.