PE Lipid DMPEC14:0 / C14:0 phosphatidylethanolamine PEG: noneHandle: custom/reactive derivativePosition: 5′ / 3′ / internal*+ Recommended oligo handle NH₂, SH, azide, DBCO, or other orthogonal handle depending on the activated DMPE derivative. Conjugation chemistry Post-synthetic amide, thiol-selective, click, or custom coupling chemistry. Design role Shorter C14 saturated tails for comparative membrane-anchor and chain-length studies.
PE LipidDPPEC16:0 / C16:0 phosphatidylethanolaminePEG: none / PEG derivativeHandle: reagent dependentPosition: 5′ / 3′ / internal*+ Design role Intermediate saturated tail length between DMPE and DSPE; useful for membrane-retention comparisons. PEG formats DPPE-PEG derivatives can extend the anchor into a surface-display architecture. Chemistry Post-synthetic conjugation through activated or click-functional PEG derivatives.
PE LipidDSPEC18:0 / C18:0 phosphatidylethanolaminePEG: none / 400–5000+Handle: multiple reactive formatsPosition: 5′ / 3′ / internal*+ Design role Strong saturated C18 membrane anchor and a widely used scaffold for PEG-lipid conjugation architectures. Reactive families NHS, maleimide, azide, DBCO, amine, COOH, PDP, TCO, tetrazine, and other supplier-dependent variants. Applications Liposomes, lipid-particle surface display, vesicles, aptamer presentation, biosensors, and membrane anchoring.
PE LipidDOPEC18:1 / C18:1 phosphatidylethanolaminePEG: none / PEG derivativeHandle: reagent dependentPosition: 5′ / 3′ / internal*+ Design role Unsaturated C18 tails provide a useful comparison against saturated DSPE for packing and membrane-assembly studies. PEG formats DOPE-PEG reactive derivatives can be used for PEG-spaced oligonucleotide display. Chemistry Post-synthetic conjugation using suitable reactive DOPE or DOPE-PEG derivatives.
DSPE-PEGDSPE-PEG400 / PEG1000Short PEG length seriesPEG: 400 / 1000Handle: supplier dependentPosition: 5′ / 3′ / internal*+ Design use Shorter membrane-to-oligo spacing for compact surface architectures. Tradeoff Lower steric separation than PEG2000/3400/5000; surface accessibility should be evaluated experimentally. Availability Reactive-end availability varies by supplier and PEG length.
FeaturedDSPE-PEG2000Core PEG-lipid oligonucleotide platformPEG: ~2000Handle: NHS / maleimide / N₃ / DBCO / otherPosition: 5′ / 3′ / internal*+ Architecture DSPE membrane anchor + PEG2000 spacer + reactive terminus + oligonucleotide handle. Recommended oligo handle NH₂ for NHS, SH for maleimide/PDP, DBCO for azide, N₃ for DBCO, tetrazine for TCO, or TCO for tetrazine. Applications Liposome and lipid-particle surface functionalization, aptamer display, imaging, membrane anchoring, biosensors, and vesicle engineering.
DSPE-PEGDSPE-PEG3400Extended PEG spacerPEG: ~3400Handle: maleimide / azide / amine / COOH / NHS*Position: 5′ / 3′ / internal*+ Design use Greater separation from the membrane than PEG2000 for surface-accessibility studies. Watch Longer PEG can alter surface density, steric environment, and particle architecture. Availability Reactive termini are supplier dependent.
DSPE-PEGDSPE-PEG5000Long PEG spacerPEG: ~5000Handle: maleimide / azide / DBCO / amine*Position: 5′ / 3′ / internal*+ Design use Extended surface presentation and shielding studies. Watch Greater PEG length is not automatically better and may reduce effective surface density or alter accessibility. Availability Reactive end groups vary by supplier.
NHSDSPE-PEG2000-NHSAmine-reactive PEG-lipidPEG: ~2000Oligo handle: NH₂Position: 5′ / 3′ / internal NH₂*+ Conjugation chemistry Amine–NHS coupling to form a stable amide linkage. Recommended oligo 5′-amino or 3′-amino oligo; selected internal amino modifiers are possible. Watch NHS ester hydrolysis and pH-sensitive coupling efficiency.
MaleimideDSPE-PEG2000-MaleimideThiol-reactive PEG-lipidPEG: ~2000Oligo handle: SHPosition: 5′ / 3′ / internal SH*+ Conjugation chemistry Thiol–maleimide coupling to form a thioether-type linkage. Recommended oligo 5′-thiol or 3′-thiol oligo; internal thiol handles can be used for selected architectures. Watch Thiol oxidation, maleimide hydrolysis, and linkage stability under the intended conditions.
SPAACDSPE-PEG2000-AzideAzide-terminated PEG-lipidPEG: ~2000Oligo handle: DBCOPosition: 5′ / 3′ / internal DBCO*+ Chemistry Copper-free SPAAC between lipid azide and DBCO-modified oligonucleotide. Advantages Bioorthogonal, no copper catalyst, compatible with multifunctional constructs. Final linkage Stable triazole-containing conjugate.
SPAACDSPE-PEG2000-DBCODBCO-terminated PEG-lipidPEG: ~2000Oligo handle: N₃Position: 5′ / 3′ / internal N₃*+ Chemistry Copper-free SPAAC between DBCO-lipid and azide-modified oligonucleotide. Advantages Excellent orthogonality for oligos that already contain amines or thiols. Final linkage Stable triazole-containing conjugate.
AmideDSPE-PEG2000-COOH / AmineCarboxyl- or amine-terminated PEG-lipidPEG: ~2000Oligo handle: NH₂ or activated COOHPosition: terminal / internal*+ COOH route Activate the lipid carboxyl group, then couple to amino-modified oligonucleotide. Amine route Couple DSPE-PEG-amine to a compatible activated carboxyl-containing oligo linker or custom reagent. Watch Activation chemistry and hydrolysis must be controlled.
PDPDSPE-PEG2000-PDPThiol-reactive disulfide-capable architecturePEG: ~2000Oligo handle: SHPosition: 5′ / 3′ / internal SH*+ Chemistry Thiol-reactive pyridyldisulfide chemistry can generate a disulfide-containing linkage. Linkage character Potentially cleavable under reducing conditions depending on the exact construct. Use Cleavable membrane-anchor or release-oriented design studies.
IEDDADSPE-PEG2000-TCOTrans-cyclooctene PEG-lipidPEG: ~2000Oligo handle: tetrazinePosition: 5′ / 3′ / internal*+ Chemistry Inverse-electron-demand Diels–Alder ligation with tetrazine-modified oligonucleotide. Advantages Fast bioorthogonal conjugation without copper. Use Complex multifunctional or rapid-ligation architectures.
IEDDADSPE-PEG2000-TetrazineTetrazine PEG-lipidPEG: ~2000Oligo handle: TCOPosition: 5′ / 3′ / internal*+ Chemistry IEDDA ligation with TCO-modified oligonucleotide. Advantages Highly selective rapid coupling for orthogonal assembly. Watch Tetrazine stability and compatibility with the full oligo architecture.
PEG-PEDPPE-PEG2000C16 saturated phospholipid PEG anchorPEG: ~2000Handle: azide / other supplier-dependentPosition: 5′ / 3′ / internal*+ Design role Useful for comparing a C16 saturated anchor against DSPE C18 saturated PEG-lipids. Chemistry Reactive-end dependent post-synthetic conjugation. Application Membrane insertion and PEG-spaced surface display.
PEG-PEDOPE-PEG2000C18:1 unsaturated PEG-phospholipidPEG: ~2000Handle: amine / azide / other*Position: 5′ / 3′ / internal*+ Design role Compare unsaturated DOPE tails against saturated DSPE for packing and insertion behavior. Chemistry Reactive-end dependent post-synthetic conjugation. Applications Lipid assembly, membrane anchoring, and surface-display research.
PEG-GlycerolipidDMG-PEG2000Related PEG-lipid anchorPEG: ~2000Handle: azide / maleimide / DBCO / TCO*Position: 5′ / 3′ / internal*+ Classification Related PEG-glycerolipid anchor rather than phosphatidylethanolamine. Chemistry Reactive-end dependent post-synthetic conjugation. Applications Lipid-particle, membrane-insertion, and surface-functionalization studies.
PEG-CeramideC8 / C16 PEG-CeramidesSpecialty sphingolipid-derived anchorsPEG: ~750 / 2000 / 5000*Handle: custom/reactive derivativePosition: custom+ Classification PEG-ceramides are distinct from PE lipids and glycerolipid PEG anchors. Design role Alternative membrane-anchor architecture for comparative insertion and assembly studies. Conjugation Project-specific reactive derivative and oligonucleotide handle selection.
GlycerolipidPhoDAG / Custom GlycerolipidsPhotoresponsive or specialty glycerolipid anchorsPEG: none / customHandle: customPosition: 5′ / 3′ / internal/custom+ Architecture Specialty glycerolipid scaffold rather than phosphatidylethanolamine. Design use Photoresponsive, membrane-interaction, or custom lipid-oligo studies. Development Requires project-specific building-block or post-synthetic conjugation strategy.