Azide C4 NHS Oligo Modification

Azide C4 NHS modified oligo is a post synthesis reaction with a reactive primary amino modified oligo. After synthesis and further purified, the azidde NHS ester is than manually attached to the oligonucleotide post-synthetically follow with step 2 purification process. This modification can be place at any position within can oligonucleotide. As a result, the alkyne group is separated from the oligo by a spacer arm of varying length, which serves to reduce steric interaction between the reactive group and the oligo.  This azide functionalized oligo can be used to attach alkyne modified biomolecule through the click reaction using copper (I) iodide as a catalyst) to conjugate the azide-modified oligo to a terminal alkyne-modified oligo, peptide or other biomolecule with extremely high regioselectivity and efficiency ^(1,2) .

Product Information

 

Product Name:

Azide C4 NHS Oligo Modification

Alternate Name:

Azide butyrate NHS

Category:

Click Chemistry

Modification Code:

[Azide-C4 NHS]

Exact Mass:

113.12

Structure:

Purification:

dual HPLC or PAGE/HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
2. Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
3. Kumar, R., El-Sagheer, A., Tumpane, J., Lincoln, P., Wilhelmsson, L.M., Brown, T. Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click Chemistry. J. Am. Chem. Soc. (2007), 129: 6859-6864.

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