DBCO Copper-Free Oligo Modification

Bio-Synthesis offer DBCO modified oligonucleotide synthesis. This dibenzocyclooctyl DBCO structure  is highly reactive towards azides without the need for copper catalysis, this modification can be place at any position within an oligonucleotide. This modification can be place in the 5' , 3' or internally.

For 5' modification, we use 5'-DBCO phosphoramidite chemistry in which the very hydrophobic DBCO moiety is separeated from the oligonucleotide with a PEG spacer. For internal DBCO oligo modification, a primary amine modified oligo is used to conjugate DBCO post-synthethically via NHS ester cross linking chemistry. Dual HPLC purification is requuire for this process.

Contact us for DBCO oligonucleotide modification services.

Please note: Bio-Synthesis does not offer Copper based Click Chemistry, we only offer Cooper free click chemistry.
For Copper based Click Chemistry please contact baseclick GMBH.

Product Information

 

Product Name:

DBCO Copper-Free Oligo Modification

Chemical Name:

Dibenzocyclooctyl

Category:

Click Chemistry

Modification Code:

[DBCO]

Structure:

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. V.V. Rostovtsev, Green, L.G., Fokin, V.V. and Sharpless, K.B., Angew Chem Int Ed, 2002, 41, 2596-2599.
2. P. van Delft, et al., Org Lett, 2010, 12, 5486-9.
3. P. van Delft, et al., Synthesis-Stuttgart, 2011, 2724-2732.
4. I.S. Marks, et al., Bioconjugate Chemistry, 2011, 22, 1259-1263.

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