Synthetic long RNA is widely used in a variety of applications ranging from understanding
the function of newly discovered genes to gene analysis, as positive controls for
any sensitive molecular diagnostic tools and the development of medical therapeutics
. However, these newly developed methods have raised the demand for even longer RNA oligonucleotides
which allows complete flexibility in the choice of sequences and the ability to
introduce modifications at specific sites. Bio-Synthesis provides a one-stop solution
to all our customers' nucleic acid technology needs. Our track record and expertise
in providing superior quality of synthetic Long RNA Oligo UbermerTM
(150 bases and more) with superior quality and services which are unmatched by other
Bio-Synthesis has optimized the synthesis and purification of RNA oligos. Every RNA oligonucleotide manufactured at Bio-Synthesis undergoes quality control analysis by PAGE, HPLC and/or MS. Additional service options including custom preparative purification and quality control, formulation, annealing, aliquoting and large batch synthesis up to 10 grams of final product.
Looking for RNA >150 bases? See Ultramer RNA Transcript Synthesis services.
While there has been significant demand for solid phase synthesis of long RNA fragments
such as miRNA, shRNA in gene structure and functional studies, the ability to synthesize
longer oligos is highly sequence-dependent due to the potential for secondary structure
formation and the inherent difficulty for chemical synthesis with additional 2'-OH
reactive group of RNA introduces considerable complexity to the synthesis. Therefore,
an effective chemical synthetic method for longer RNA is an important basic technology
for further advancement on RNA related research.
Bio-Synthesis has met the challenge of synthesizing thousands of synthetic long
RNA, RNA UbermerTM up to 200 bases and d2RNATM Long Oligos
(3000 bases and more) using the best reagents available, proprietary methods to
optimized synthesis protocols and state-of-art synthesis facilities and expertise
in providing oligonucleotide synthesis services since 1984.
Each RNA oligo is meticulously monitored during synthesis and controlled according
to Bio-Synthesis’s stringent quality assurance and quality control standards. The
final product is identified by LC-MS Electrospray, sequencing or other custom analytical
methods. Purity is analyzed by high throughput capillary gel or PAGE electrophoresis.
For over 25 years Bio-Synthesis has maintained these gold standards for long RNA
oligonucleotide synthesis that saves the researcher a great deal of time and trouble
for applications that require direct synthesis of the entire target fragment such
These long oligos can be used in variety of molecular biology application including
but not limited to:
All Bio-Synthesis oligos include desalting and deprotection at no additional charge.
We strongly recommend oligonucleotide longer than 50 bases to be purified by RNase
free poly acrylamide gel electrophoresis (PAGE). If you are unsure about the purification
level you need for your research, see our technical bulletin titled
What type of purification I need for my applications or
contact us for assistance.
Unlike other suppliers, there is no additional charge for RNA Oligo UbermerTM
Yield will vary based upon base composition, purification, oligo length, scale and
modifications. That's why we have a set guaranteed quantity.
Oligos are routinely checked for length, purity, and composition. Long oligos are
typically quality checked by PAGE, ESI-MS, and/or CE. Our global quality control
capabilities include MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry
(ESI-MS), capillary electrophoresis (CE), and polyacrylamide gel electrophoresis
(PAGE). If you are dissatisfied and report an oligo problem within 30 days of order
receipt, we will gladly remake your oligo free of charge or provide a credit for
the purchase amount.
All orders are shipped with a Quality Assurance document that includes measured
yields, melting temperature, MW and µg/OD with every oligo.
Bio-Synthesis provides synthesis of oligo ultramer and modification with flexible
synthesis scales for your individual research needs. The ability to synthesize RNA
is critical to many laboratory techniques. Radiolabled and nonisotopically labeled
RNA probes can be used in blot hybridization and nuclease protection assays. Modified
RNA can be used for various biochemical and molecular biology studies. RNA can also
use as control for gene expression analysis (microinjection, infection with viral
transcripts, in vitro translation), structure analysis (protein-RNA binding), and
RNA -based diagnostic testing and drug development.
Contact us for large scale synthesis.
Contact us for modification not
listed above, in most cases we can help!