PC Biotin Oligonucleotide Modification
Bio-Synthesis provides photocleavable PC biotin oligonucleotide for biotin applications to capture oligonucleotides or PCR products from a crude mixture and then to release them in a pure biologically active form. Biotin-streptavidin interaction is irreversible. This ineraction is stable over a broad pH and temperature specturm. Conditions necessary to release biotin can be potentially harmful or negatively affect downstream application. PC Biotin contained a photocleavable spacer arm which can be cleaved when exposed to UV light at specific wavelength (300-350 nm)1. Moreover, upon cleavage, the resulting DNA oligo will have a free phosphate group available for subsequent ligase reactions. Contact us for PC-Biotin Modified Oligonucleotide Synthesis Services.
Product Information
PC Biotin Oligonucleotide Modification
Biotin, Photocleavable, Affinity Ligand
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- J. Olejnik, S. Sonar, E. Krzymanska-Olejnik, and K.J. Rothschild, Proc Natl Acad Sci U S A, 1995, 92, 7590-4.
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