Enhanced Diagnostic Tools
Bio-Synthesis offer caged oligonucleotide synthesis and labeling. Photocaging has developed into an attractive and simple procedure for achieving exquisite control over complex biological processes through photoactivation by UV light. The term ‘caging’ refers to the attachment of a photolabile protecting group to a biologically active molecule at specific locations, effectively rendering the molecule inactive. After irradiation by light at the wavelength required to remove the protecting group, the biological properties of the ‘uncaged’ molecule are restored. Caged oligonucleotide has been used in PCR and polymerase activity1, antisense and gene silencing2, regulartion of restriction endonuclease activity3, and enzyme-free mutagenesis4 . Caged oligonucleotide also been used to activation and deactivation of DNAzyme and antisense activity controlling gene expression in mammalian cells5, and photochemical control of DNA decoy function to regulate gene transcription6. Contact Contact Bio-Synthesis for Fluorescent base modified oligonucleotides.
Bio-Synthesis caged modified bases can be incorporate at any position of an oligonucleotide . Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
Quality Control: Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry
Delivery times: 5-7 Working days
Shipping conditions: Room temperature
Storage conditions: -20 oC to -70 oC
Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.