Enhanced Diagnostic Tools
Bio-Synthesis is a provider of Custom Peptide Synthesis for the life science research community since 1984. We offer a complete range of peptide synthesis services ranging from bulk API peptides to high throughput library peptides and array peptide preparation. Custom Peptides Synthesis is performed to produce research-grade or bulk API polypeptides. We specialize in both, liquid phase and solid phase Fmoc-chemistry. Liquid Phase Peptide Synthesis is used for the synthesis of very short peptides such as dipeptides, while large-scale Solid Phase Peptide Synthesis is well suited for preparing biologically active peptides including long, difficult and hydrophobic peptides. Bio-Synthesis also provides a large number of peptide modifications as well as custom peptides for diagnostic and therapeutic applications.
For additional information, please contact us or send an email to firstname.lastname@example.org
Visit our Catalog Peptides page for a full list of our catalog peptides
Peptides are available from immunograde purity (> 70 %, sufficient for generating or testing antibodies) to higher purity (+ 95 %, required in enzymological and biological activity studies and other applications). Peptides can be synthesized up to 200 amino acids. Longer peptides can be made through recombinant peptide technology.
We offer hundreds of peptide modifications, labels, and bioconjugation to other biomolecules, including:
We also offer a wide range of modified and special amino acids with peptide mix of 2-19 random amino acids, and peptide-D-form.
Bio-Synthesis provides end to end peptide solutions. From peptide synthesis, modification, labeling and conjugation to a wide range of biopolymers and surface attachments.
Extended Preparative Services
Bio-synthesis uses liquid phase and solid phase Fmoc-based peptide chemistries in a state-of-the-art platform allowing for rapid synthesis of peptides in large and high throughput quantities with a streamlined production and fully automated instrumentation set-up. After the completion of synthesis, peptides are cleaved from the resin and de-protected using standard TFA based cleavage cocktails. Peptides are precipitated from the cleavage cocktail using cold diethyl ether. The precipitate is subsequently washed with cold ether and dissolved in a buffer or solution allowing for efficient purification and lyophilization. Most custom peptides are purified by HPLC using reverse phase C4 and C18 columns. Alternative purification methods (e.g., ion exchange and gel filtration chromatography) are also available and used when needed. To achieve a highly pure product, peptides are purified by preparative reverse phase HPLC, freeze dried and packaged before shipping. QC is done using analytical liquid chromatography and mass analysis by MALDI-MS is provided for every peptide to ensure the highest quality of the final product.
Peptides are smaller than proteins but often contain part of a protein's sequence. As such, they can be used as research tools to study their effects in biological systems. Specifically, peptides may function as signaling molecules via their interaction with specific receptors, for example as hormones or cytokines. Also, peptides can be used for therapeutic purposes.
The following is a list of areas of research for synthetic peptides.
Bio-Synthesis can assist customers in selecting the appropriate peptide sequence, purity and quantity appropriate to your needs. Sequences are analyzed for hydrophobicity at pH 2 and pH 6.8, aggregation potential, and other known chemistry issues that may arise due to the peptide's amino acid composition including a hydrophobicity/hydrophilicity plot (Hoop-Woods and Kyte-Doolittle algorithms). For antibody production, we include antigenic index (Jameson-Wolf) and surface probability evaluation. We provide feedback on the feasibility of the candidate sequences with respect to stability, solubility and price. The customer is notified of any potential issues ahead of time so that sequence modifications can be made before starting synthesis. Our peptide design tool provides analysis output for peptide sequences. If specifications of the order are not achieved, the customer is notified immediately and given advice for redesigning the sequence or exploring alternative chemistries.
When requesting a quote, a peptide assessment, or to place an order, please specify the needed amount and the desired purity level. If you need assistance, just ask us, we are here to help. Please note that your sequence information is always kept in strict confidence.
Each peptide is shipped with a Certificate of Analysis which includes details of the peptide, the quantity and purity supplied, and helpful information on the solubility of the peptide in the buffers used during purification. We also provide a printout of the analytical HPLC profile and MALDI-TOF-MS or electrospray MS data. Our peptides are packed under dry argon gas to minimize oxidation or other types of degradation during shipment and storage. After opening, we recommend that peptides shall be stored dry in a freezer.
All peptides are analyzed by MALDI Mass Spectrometry to determine the molecular weight of the synthesized peptide. Comparison of the theoretical molecular weight with the determined molecular weight confirms the absence of amino acid deletion or double coupling. MALDI is an excellent method for determining the presence of the desired product but is inadequate for the determination of purity. Due to the variant efficiencies of peptide ionization in the MALDI instrument, peak intensities in MALDI spectra are not necessarily quantitative. For this reason all non-crude peptides are analyzed by Reverse-Phase HPLC (RP-HPLC) in order to determine purity. Additional QC analysis such as amino acid analysis, NMR, CE, as well as peptide content determination may be performed to confirm the quality of custom peptides.
Successful synthesis depends on the sequence of the amino acids. Likewise, the coupling efficiency of amino acids in the sequence and ultimate purity of the synthesized product is also related to the sequence. Impurities such as salts, deletion peptides (created due to incomplete coupling), protecting groups and modified peptides can range from 10-50%.
Purification of synthetic peptides is carried out by HPLC using reverse phase C4 and C18 columns. Alternate purification methods (e.g., ion exchange and gel filtration chromatography) are used as needed. For purity requirements of >95%, we conduct the HPLC analysis at a gradient of 0-60% over a period of 45 minutes. This insures that peptide validation reveals even the smallest of impurities. Peptides meeting or exceeding the purity requirements are checked again by MALDI or additional extended quality control or preparative services.
We constantly seek ways to improve our quality systems and welcome any suggestions from our customers. Our commitment to quality is ongoing throughout the manufacturing process. The process begins with raw material quality inspection (RMQ), in-process quality inspection (IPQ) and on to final product quality inspection (FPQ) which are subject to a battery of analytical tests. Each peptide is accompanied with a certificate of analysis which includes details of the peptide, quantity and purity supplied, and helpful information on the solubility of the peptide in the buffers used during purification. Purified peptides also include a printout of the analytical HPLC profile and electrospray MS data. Peptides are also shipped with "A guide to handling peptides" and "Interpreting the results of peptide analysis".
Peptides are packed under dry argon gas to minimize oxidation or other types of degradation during shipment and storage. After opening, we recommend that peptides be stored dry in a freezer (details given in our technical guide to handling peptides; a copy is shipped with each peptide).
We offer a variety of peptide synthesis services . From micro to macro high throughput peptide arrays and peptide library synthesis to specialty peptide modifications, ultra long peptides, high quality bulk API peptides and over 10,000 ready to ship catalog peptides.
Peptides from micro-gram to multi-gram at various length, purities and modifications. Delivered in 5-7 days.
Wide spectrum of modifications from simple dye labeling to complex structure modifications.
High-fidelity synthesis of ultra long peptides up to small proteins.
Cross-linking peptides to oligos, antibodies, protein beads, plates, glass and various solid supports.
High throughput peptide synthesis of a systematic combination of different peptides.
Large, cost-effective sets of peptides formatted to your needs.
Multi-grams available at affordable prices.
Powerful recombinant peptide technologies for peptides in any scale and length.
Integrated CRO solutions. Providing custom peptides, peptidomimetics and proteins to the life science communities.
Sample preparation, peptide/ protein characterization, ID, quantification and biomarker services.
Bio-Synthesis is a leading supplier of bioactive synthetic peptides, enzyme substrates and inhibitors.
Bio-Synthesis offers a broad range of products and services for life science research in genomics.
Over 10,000 pre-made high quality peptide lyophilized aliquots available for your immediate experimental needs.
High quality. ready-to-go, research grade cosmetic peptides.
For detection and separate antigen specific CD8+T cell population, suitable for epitope mapping and immune monitoring.
Peptide pools and libraries in 15 mer peptides overlapping by 11 amino acids. 25µg of >70% purity and ready to use.
Synthetic polypeptides have spurred the development of different applications in biochemistry, molecular biology, immunology and medicine. Synthetic peptides may be used in epitope-specific antibodies against pathogenic proteins, the study of protein functions and identification and characterization of proteins. Furthermore, synthetic peptides are used to study enzyme-substrate interactions within important enzyme classes such as kinases and proteases, which play a crucial role in cell signaling.
In diagnostics, a set of homologous synthetic peptide binding ligands would be useful in cases such as directing reagents to particular epitopes in a protein, detection of peptide hormones, and many other applications. In therapeutic applications, peptide drugs are either resembled as naturally-occurring peptides or as altered natural peptides. If the analog is more biochemically active than the original peptide, it is known as an agonist and if it has the reverse effect is known as an antagonist.
Synthetic peptides are used as standards and as reagents in mass spectrometry (MS)-based applications. Synthetic peptides play a central role in MS-based discovery, characterization and quantitation of proteins, especially those that serve as early biomarkers for diseases.
Bio-Synthesis offers a broad range of products and services for life science research in genomics.
Biomarker Discovery, Epitope Validation, Immuno Monitoring
Screening & Discovery, Research Discovery Peptides, pep-CHIP
Spiked Isotope Peptides, Peptide-in-Plate (96 well plates), pep-CHIP Microarrays
Peptide substrate for kinase, protease and phosphatase profiling in a variety of formats
Peptide/Protein Identification, Peptide/Protein Quantification, Peptide Protein Characterization