After synthesis, all crude oligo synthesize by BSI are desalted using size exclusion desalting column to remove the protecting groups and other synthesis by-products.
Since the most efficient coupling success rate (average 99%), about 1-2% of all extension fails, resulting the relative proportion of undesired (n-x) material increase with the length of oligonucleotides, purification to obtain full length oligonucleotides may required for some specific applications. Bio-Synthesis offers three types of purifications 1) HPLC 2) PAGE 3) RP-Cartridge.