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Example 1 of BNA probe:
C+TGG+C CG+TCG +TGG+CC C+GCA
Estimated melting temperature: 87 to 90 °C (DNA-Tm) / 90 to 96 °C (RNA-Tm).
Example 2 of BNA probe:
C+T+GGC CG+TCG +TGG+CC C+G+CA
Less modifications are needed if a lower melting temperature is desired.
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