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Methods of Purification for RNA Transcript Synthesis

Bio-Synthesis offers custom RNA synthesis by transcription. In a transcription reaction, an RNA polymerase produces many copies of RNA by transcribing a DNA template which encodes the RNA. After RNA synthesis, we perform two purification process. First, phenol-chloroform extraction and ethanol precipitation. This method is use to remove proteins and most of the free nucleotides. Second, Spin column chromatrography can be used to remove additional unincorporated nucleotides, proteins and salts. For applications such as labeled RNA probes for RNase protection assay or foot printing experiments which require extremely pure RNA transcript, we recommend gel purification as an optional service.

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Trusted by biotech leaders worldwide for over 45 years of delivering high-quality, fast, and scalable synthetic biology solutions.

Greetings, Researchers,

Please be advised that any information, guidelines, write-ups, and oral/video/graphic content on our website are intended solely as general guidelines.

It is the researcher's sole responsibility to conduct thorough research on the design of products intended for their experiments before submitting
their designs to Biosynthesis for production feasibility review.

Thank you for your understanding.

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