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Oligo Tm calculation

Bio-Synthesis offers a free online oligo Tm calculation, which will determine the Tm of your oligonucleotide sequence for your specifici reaction. Access the tool at http://www.biosyn.com/SciApps.aspx. Be sure to include the Mg2+ and dNTP concentrations that you plan to use in your reaction, and specify whether hybridization is to DNA or RNA targets, as these will affect the Tm of your oligo.

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Greetings, Researchers,

Please be advised that any information, guidelines, write-ups, and oral/video/graphic content on our website are intended solely as general guidelines.

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their designs to Biosynthesis for production feasibility review.

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