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Fluorochrome Absorption Emission Wavelengths

What are the fluorochrome absorption emission wavelengths?

 
Fluorochrome Absorption (nm) Emission (nm) Other info
1,5 IAEDANS 336 490  
1,8-ANS 372 480  
4-Methylumbelliferone 385 502  
5-carboxy-2,7-dichlorofluorescein 504 529  
5-Carboxyfluorescein (5-FAM) 492 518  
5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH
5-Carboxytetramethylrhodamine (5-TAMRA) 542 568  
5-FAM (5-Carboxyfluorescein) 492 518  
5-HAT (Hydroxy Tryptamine) 370-415 520-540  
5-Hydroxy Tryptamine (HAT) 370-415 520-540  
5-ROX (carboxy-X-rhodamine) 578
567		 604
591		  
5-TAMRA (5-Carboxytetramethylrhodamine) 548
542		 552
568		  
6-Carboxyrhodamine 6G 518 543  
6-CR 6G 518 543  
6-JOE 520 548  
7-Amino-4-methylcoumarin 351 430  
7-Aminoactinomycin D (7-AAD) 546 647  
7-Hydroxy-4-methylcoumarin 360 449, 455  
9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474  
ABQ 344 445  
Acid Fuchsin 540 630  
ACMA (9-Amino-6-chloro-2-methoxyacridine) 412, 430 471, 474  
Acridine Orange + DNA 502 526  
Acridine Orange + RNA 460 650  
Acridine Orange, both DNA & RNA 440-480 520-650  
Acridine Red 455-600 560-680  
Acridine Yellow 470 550  
Acriflavin 436 520  
Acriflavin Feulgen SITSA 355-425 460  
Aequorin (Photoprotein)   466 Photoprotein
AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP      
Alexa Fluor 350™ 346
342		 442
441		  
Alexa Fluor 430™ 431 540  
Alexa Fluor 488™ 495, 492 519, 520  
Alexa Fluor 532™ 531, 532 553, 554  
Alexa Fluor 546™ 556, 557 572, 573  
Alexa Fluor 568™ 577, 578 603  
Alexa Fluor 594™ 590, 594 617, 618  
Alexa Fluor 633™ 632 650  
Alexa Fluor 647™ 647 666  
Alexa Fluor 660™ 668 698  
Alexa Fluor 680™ 679 702  
Alizarin Complexon 530-560, 580 580, 624-645  
Alizarin Red 530-560 580  
Allophycocyanin (APC) 630, 645 655, 660  
AMC, AMCA-S 345 445  
AMCA (Aminomethylcoumarin) 345
347		 425
444		  
AMCA-X 353 442  
Aminoactinomycin D 555 655  
Aminocoumarin 346
350		 442
445		  
Aminomethylcoumarin (AMCA) 345
347		 425
444		  
Anilin Blue   600  
Anthrocyl stearate 360-381 446  
APC (Allophycocyanin) 630, 645 655, 660  
APC-Cy7 625-650 755  
APTRA-BTC = Ratio Dye, Zn2+ 466/380 520/530 Ratio Dye, Zn2+
APTS 424 505  
Astrazon Brilliant Red 4G 500 585  
Astrazon Orange R 470 540  
Astrazon Red 6B 520 595  
Astrazon Yellow 7 GLL 450 480  
Atabrine 436 490  
ATTO-TAG™ CBQCA 465 560  
ATTO-TAG™ FQ 486 591  
Auramine 460 550  
Aurophosphine G 450 580  
Aurophosphine 450-490 515  
BAO 9 (Bisaminophenyloxadiazole) 365 395  
BCECF (high pH) 492, 503 520,528  
BCECF (low pH) 482 520  
Berberine Sulphate 430 550  
Beta Lactamase 409 447, 520  
BFP blue shifted GFP (Y66H)
Blue Fluorescent Protein		 381, 382, 383 445, 447, 448 blue shifted GFP (Y66H)
Blue Fluorescent Protein
BFP/GFP FRET      
Bimane 398 490  
Bisbenzamide 360 461  
Bisbenzimide (Hoechst) 360 461  
bis-BTC = Ratio Dye, Zn2+ 455/405 529/505 Ratio Dye, Zn2+
Blancophor FFG 390 470  
Blancophor SV 370 435  
BOBO™ -1 462 481  
BOBO™ -3 570 602  
Bodipy 492/515 490 515  
Bodipy 493/503 533 549  
Bodipy 500/510 509 515  
Bodipy 505/515 502 510  
Bodipy 530/550 528 547  
Bodipy 542/563 543 563  
Bodipy 558/568 558 569  
Bodipy 564/570 564 570  
Bodipy 576/589 579 590  
Bodipy 581/591 584 592  
Bodipy 630/650-X 625 642  
Bodipy 650/665-X 647 665  
Bodipy 665/676 605 676  
Bodipy Fl 504, 505 511, 513  
Bodipy FL ATP 505 514  
Bodipy Fl-Ceramide 505 511  
Bodipy R6G SE 528 547  
Bodipy TMR 542 574  
Bodipy TMR-X conjugate 544 573  
Bodipy TMR-X, SE 544 570  
Bodipy TR 589 617  
Bodipy TR ATP 591 620  
Bodipy TR-X SE 588 616  
BO-PRO™ -1 462 481  
BO-PRO™ -3 544 570  
Brilliant Sulphoflavin FF 430 520  
BTC - Ratio Dye Ca2+ 464/401 533/529 Ratio Dye Ca2+
BTC-5N - atio Dye, Zn2+ 459/417 517/532 Ratio Dye, Zn2+
Calcein 494 517  
Calcein Blue 373 440  
Calcium Crimson™ 588, 589 611, 615  
Calcium Green 501, 506 531  
Calcium Green-1 Ca2+ Dye 506 531 Ca2+ Dye
Calcium Green-2 Ca2+ 506/503 536 Ca2+
Calcium Green-5N Ca2+ 506 532 Ca2+
Calcium Green-C18 Ca2+ 509 530 Ca2+
Calcium Orange 549 575
576		  
Calcofluor White 385, 395, 405 437, 440, 445  
Carboxy-X-rhodamine (5-ROX) 576 601  
Cascade Blue™ 377
398
399		 420
423		  
Cascade Yellow 399
400		 550
552		  
Catecholamine 410 470  
CCF2 (GeneBlazer)      
CFDA 494 520  
CFP - Cyan Fluorescent Protein 430, 433, 436, (453) 474, 475, 476, (501) Cyan Fluorescent Protein
CFP/YFP FRET      
Chlorophyll 480 650  
Chromomycin A 436-460 470  
Chromomycin A 445 575  
CL-NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pH
CMFDA 494 520  
Coelenterazine Ca2+ Dye, bioluminescence (429) 465 Ca2+ Dye, bioluminescence,
native molecule
Coelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+ Dye, bioluminescence
Coelenterazine f (437) 473 Ca2+ Dye, bioluminescence
Coelenterazine fcp   452 Ca2+ Dye, bioluminescence
Coelenterazine h (437) 464 Ca2+ Dye, bioluminescence
Coelenterazine hcp (433) 444 Ca2+ Dye, bioluminescence
Coelenterazine ip   441 Ca2+ Dye, bioluminescence
Coelenterazine n (431) 467 Ca2+ Dye, bioluminescence
Coelenterazine O 460 575  
Coumarin Phalloidin 387 470  
C-phycocyanine      
CPM Methylcoumarin 384 469 Methylcoumarin
CTC 400-450 602  
CTC Formazan      
Cy2™ 489 506  
Cy3.1 8 554 568  
Cy3.5™ 581 598  
Cy3™ 514
552
554		 566
570		  
Cy5.1 8 649 666  
Cy5.5™ 675 695  
Cy5™ 649 666
670		  
Cy7™ 710, 743 767, 805  
Cyan GFP 433 (453) 475 (501)  
cyclic AMP Fluorosensor (FiCRhR) 500 517  
CyQuant Cell Proliferation Assay 480 520 Cell Proliferation Assay
Dabcyl 453    
Dansyl 340 578  
Dansyl Amine 337 517  
Dansyl Cadaverine 335 518  
Dansyl Chloride 372 518  
Dansyl DHPE 336 517  
Dansyl fluoride 356 none  
DAPI 359 461  
Dapoxyl 403 580  
Dapoxyl 2 374 574  
Dapoxyl 3 373 574  
DCFDA 504 529  
DCFH (Dichlorodihydrofluorescein Diacetate) 505 535  
DDAO 463 607  
DHR (Dihydorhodamine 123) 505 534  
Di-4-ANEPPS 496 705  
Di-8-ANEPPS (non-ratio) 488
498		 605
713		  
DiA (4-Di-16-ASP) 456 591  
Dichlorodihydrofluorescein Diacetate (DCFH) 505 535  
DiD - Lipophilic Tracer 644 665 Lipophilic Tracer
DiD (DiIC18(5)) 644 665  
DIDS 341 415  
Dihydorhodamine 123 (DHR) 505 535  
DiI (DiIC18(3)) 549, 551 565  
Dinitrophenol 349    
DiO (DiOC18(3)) 484, 487 501, 502  
DiR 748 780 Lipophilic Tracer
DiR (DiIC18(7)) 750 779  
DM-NERF (high pH) 497/510 540 Ratio Dye, pH
DNP 349    
Dopamine 340 490-520  
DsRed 558 583 Red fluorescent protein
DTAF 494 520  
DY-630-NHS 621 660 Hemicyane
label for proteins and DNA
DY-635-NHS 634 664 Hemicyane
label for proteins and DNA
EBFP 383 447 Enhanced Blue Fluorescent Protein
ECFP 436 474 Enhanced Cyan Fluorescent Protein
EGFP 488, 498 507, 516 Enhanced Green Fluorescent Protein
ELF 97 345 530  
Eosin 524 545  
Erythrosin 529, 532 554, 555  
Erythrosin ITC 529 555  
Ethidium Bromide 510, 523 595, 605  
Ethidium homodimer -1 (EthD-1) 528 617  
Euchrysin 430 540  
EukoLight      
Europium (III) chloride      
EYFP 513, 520 527, 532 Enhanced Yellow Fluorescent Protein
Fast Blue 360 440  
FDA 494 520  
Feulgen (Pararosaniline) 570 625  
FIF (Formaldehyd Induced Fluorescence) 405 433  
FITC 490, 494 520, 525  
FITC Antibody 493 517  
Flazo Orange 375-530 612  
Fluo-3 480 – 506, 506 520, 527  
Fluo-4 494 516  
Fluorescein (FITC) 490, 494 520, 525  
Fluorescein Diacetate 494 520  
Fluoro-Emerald 495 524  
Fluoro-Gold (Hydroxystilbamidine) 361 536  
Fluor-Ruby 555 582  
FluorX 494 520  
FM 1-43™ 479 598  
FM 4-46 515 640  
Fura Red™ (high pH) 572 657  
Fura Red™/Fluo-3      
Fura-2, high calcium 335 505 Excitation ratio dye
Fura-2, low calcium 363 512 Excitation ratio dye
Fura-2/BCECF      
Genacryl Brilliant Red B 520 590  
Genacryl Brilliant Yellow 10GF 430 485  
Genacryl Pink 3G 470 583  
Genacryl Yellow 5GF 430 475  
GeneBlazer (CCF2)      
GFP (S65T) 498 516  
GFP red shifted (rsGFP) 498 516  
GFP wild type, non-UV excitation (wtGFP) 475 509  
GFP wild type, UV excitation (wtGFP) 395 509  
GFPuv 385 508  
Gloxalic Acid 405 460  
Granular Blue 355 425  
Haematoporphyrin 530-560 580  
Hoechst 33258 345 487  
Hoechst 33342 347 483  
Hoechst 34580 392 440  
HPTS 355 465  
Hydroxycoumarin 325-360 386-455  
Hydroxystilbamidine (FluoroGold) 361 536  
Hydroxytryptamine 400 530  
Indo-1, high calcium 330 401 Emission ratio dye
Indo-1, low calcium 346 475 Emission ratio dye
Indodicarbocyanine (DiD) 644 665  
Indotricarbocyanine (DiR) 748 780  
Intrawhite Cf 360 430  
JC-1 514 529  
JO-JO-1 530 545  
JO-PRO-1 532 544  
LaserPro 795 812  
Laurodan 355 460  
LDS 751 (DNA) 543 712  
LDS 751 (RNA) 590 607  
Leucophor PAF 370 430  
Leucophor SF 380 465  
Leucophor WS 395 465  
Lissamine Rhodamine 572, 577 591, 592  
Lissamine Rhodamine B 577 592  
LIVE/DEAD Kit Animal Cells
Calcein/Ethidium homodimer		 494
528		 517
617		 for more details refer
to www.probes.com
LOLO-1 566 580  
LO-PRO-1 568 581  
Lucifer Yellow 425, 428 528, 536, 540  
Lyso Tracker Blue 373 422  
Lyso Tracker Blue-White 466 536  
Lyso Tracker Green 504, 534 511, 551  
Lyso Tracker Red 490 516  
Lyso Tracker Yellow 551 576  
LysoSensor Blue 374 424  
LysoSensor Green 442 505  
LysoSensor Yellow/Blue 384 540  
Mag Green 507 531  
Magdala Red (Phloxin B) 524 600  
Mag-Fura Red 483/427 659/631 Ratio Dye, Mg2+
Mag-Fura-2 369/329
369/330		 508
511/491		 Ratio Dye Ca2+
Ratio Dye Mg2+
Mag-Fura-5 369/330
369/332		 505/500
505/482		 Ratio Dye, Ca2+
Ratio Dye, Mg2+
Mag-Indo-1 349/328
349/330		 480/390
480/417		 Ratio Dye, Ca2+
Ratio Dye, Mg2+
Magnesium Green 506, 507 531  
Magnesium Orange 550 575  
Malachite Green 628    
Marina Blue 362 459  
Maxilon Brilliant Flavin 10 GFF 450 495  
Maxilon Brilliant Flavin 8 GFF 460 495  
Merocyanin 555 578  
Methoxycoumarin 360 410  
Mitotracker Green FM 490 516  
Mitotracker Orange 551 576  
Mitotracker Red 578 599  
Mitramycin 450 470  
Monobromobimane 398 490  
Monobromobimane (mBBr-GSH) 398 500  
Monochlorobimane 380 461  
MPS (Methyl Green Pyronine Stilbene) 364 395  
NBD 466 539  
NBD Amine 450 530  
Nile Red 515-555, 559 590, 640  
Nitrobenzoxadidole 465 510-650  
Noradrenaline 340 490-520  
Nuclear Fast Red 289-530 580  
Nuclear Yellow 365 495  
Nylosan Brilliant Iavin E8G 460 510  
Oregon Green 503 522  
Oregon Green 488-X 494 517  
Oregon Green™ 503 522  
Oregon Green™ 488 490, 493 514, 520  
Oregon Green™ 500 497 517  
Oregon Green™ 514 506 526  
Pacific Blue 405 455  
Pararosaniline (Feulgen) 570 625  
PBFI 340/380 420 Excitation ratio dye
PE-Cy5 488 670  
PE-Cy7 488 755, 767  
PerCP 488 675  
PerCP-Cy5.5 488 710  
PE-TexasRed [Red 613] 488 613  
Phloxin B (Magdala Red) 524 600  
Phorwite AR 360 430  
Phorwite BKL 370 430  
Phorwite Rev 380 430  
Phorwite RPA 375 430  
Phosphine 3R 465 565  
PhotoResist 365 610  
Phycoerythrin B [PE] 546-565 575  
Phycoerythrin R [PE] 565 578  
PKH26 (Sigma) 551 567  
PKH67 496 520 Chroma
PMIA 341 376  
Pontochrome Blue Black 535-553 605  
POPO-1 433 457  
POPO-3 533 574  
PO-PRO-1 435 455  
PO-PRO-3 539 567  
Primuline 410 550  
Procion Yellow 470 600  
Propidium Iodid (PI) (305), 536, 538 617  
PyMPO 412, 415 561, 564, 570  
Pyrene 360 387  
Pyronine 410 540  
Pyronine B 540-590 560-650  
Pyrozal Brilliant Flavin 7GF 365 495  
QSY 7 560    
Quinacrine Mustard 440 510  
Red 613 [PE-TexasRed] 488 613  
Resorufin 571 584, 585  
RH 414 532 716  
Rhod-2 552 576  
Rhodamine 550 573  
Rhodamine 110 496, 497 520  
Rhodamine 123 507 529  
Rhodamine 5 GLD 470 565  
Rhodamine 6G 525 555  
Rhodamine B 540 625  
Rhodamine B 200 523-557 595  
Rhodamine B extra 550 605  
Rhodamine BB 540 580  
Rhodamine BG 540 572  
Rhodamine Green 502 527  
Rhodamine Phallicidine 558, 542 575, 565  
Rhodamine Phalloidine 542 565  
Rhodamine Red 570 590  
Rhodamine WT 530 555  
Rose Bengal 525, 540 550-600  
R-phycocyanine      
R-phycoerythrin (PE) 565 578  
rsGFP 473 509 red shifted GFP (S65T)
S65A 471 504  
S65C 479 507  
S65L 484 510  
S65T 488 511  
Sapphire GFP 395 511  
SBFI 340/380 420 Excitation ratio dye
Serotonin 365 520-540  
Sevron Brilliant Red 2B 520 595  
Sevron Brilliant Red 4G 500 583  
Sevron Brilliant Red B 530 590  
Sevron Orange 440 530  
Sevron Yellow L 430 490  
sgBFP™ 387 450  
sgBFP™ (super glow BFP) 387 450 Quantum's SuperGlo™GFP
AFPs
sgGFP™ 474 488  
sgGFP™ (super glow GFP) 474 509 Quantum's SuperGlo™GFP
AFPs
SITS 336 436 Ion Channels
SITS (Primuline) 395-425 450  
SITS (Stilbene Isothiosulphonic Acid) 365 460  
SNAFL calcein 506/535 535/620 Ratio Dye, pH
SNAFL-1 508/540 543/623 Ratio Dye, pH
SNAFL-2 514/543 546/630 Ratio Dye, pH
SNARF calcein 552/574 590/629 Ratio Dye, pH
SNARF1 576/548 635/587 Excitation and emission ratio dye
Sodium Green 506, 507 532 Na+, K+
SpectrumAqua 433,/53 480/55 Vysis
SpectrumGreen 497/30, 509/31 538/44, 524/56 Vysis
SpectrumOrange 559/38, 560 588/48 Vysis
Spectrum Red 587, 587/35 612, 612/51  
SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium) 344 443  
Stilbene 335 440  
Sulphorhodamine B can C 520 595  
Sulphorhodamine G Extra 470 570  
SYTO 11 508, 510 527, 530 Dye for DNA, RNA
SYTO 12 499, 500 522, 519 Dye for DNA, RNA
SYTO 13 488, 491 509, 514 Dye for DNA, RNA
SYTO 14 517, 521 549, 547 Dye for DNA, RNA
SYTO 15 516, 518 546, 555 Dye for DNA, RNA
SYTO 16 488, 494 518, 525 Dye for DNA, RNA
SYTO 17 621 634 Dye for DNA
SYTO 18 490, 493 507, 527 Dye for DNA, RNA
SYTO 20 512 530 Dye for DNA
SYTO 21 494 517 Dye for DNA
SYTO 22 515 535 Dye for DNA
SYTO 23 499 520 Dye for DNA
SYTO 24 490 515 Dye for DNA
SYTO 25 521 556 Dye for DNA
SYTO 40 420 441 Dye for DNA
SYTO 41 430 454 Dye for DNA
SYTO 42 433 460 Dye for DNA
SYTO 43 436 467 Dye for DNA
SYTO 44 446 471 Dye for DNA
SYTO 45 452 484 Dye for DNA
SYTO 59 622 645 Dye for DNA
SYTO 60 652 678 Dye for DNA
SYTO 61 628 645 Dye for DNA
SYTO 62 652 676 Dye for DNA
SYTO 63 657 673 Dye for DNA
SYTO 64 599 619 Dye for DNA
SYTO 80 531 545 Nucleic Acid Stain
SYTO 81 530 544 Nucleic Acid Stain
SYTO 82 541 560 Nucleic Acid Stain
SYTO 83 543 559 Nucleic Acid Stain
SYTO 84 567 582 Nucleic Acid Stain
SYTO 85 567 583 Nucleic Acid Stain
SYTOX Blue 445 470 Nucleic Acid Stain
SYTOX Green 504 523 Nucleic Acid Stain
SYTOX Orange 547 570 Nucleic Acid Stain
Tetracycline 390-425 525-560  
Tetramethylrhodamine (TRITC) 555 576  
Texas Red™ 595 620  
Texas Red-X™ conjugate 595 615  
Thiadicarbocyanine (DiSC3) 651, 653 674, 675  
Thiazine Red R 596 615  
Thiazole Orange 510 530  
Thioflavin 5 430 550  
Thioflavin S 430 550  
Thioflavin TCN 350 460  
Thiolyte 370-385 477-488  
Thiozole Orange 453 480  
Tinopol CBS (Calcofluor White) 390 430  
TMR 550 573  
TO-PRO-1 515 531  
TO-PRO-3 644 657  
TO-PRO-5 747 770  
TOTO-1 514 531, 533  
TOTO-3 642 660  
TriColor (PE-Cy5) (488) 650 667  
TRITC TetramethylRodamineIsoThioCyanate 550 573  
True Blue 365 425  
TruRed 490 695  
Ultralite 656 678  
Uranine B 420 520  
Uvitex SFC 365 435  
wt GFP 395 (475) 508 wild type GFP
WW 781 605 639  
X-Rhodamine 580 605  
XRITC 582 601  
Xylene Orange 546 580  
Y66F 360 508  
Y66H 360 442  
Y66W 436 485  
Yellow GFP 513 527 Yellow shifted
Green Fluorescent Protein
YFP 513, 520 527, 532 Yellow Fluorescent Protein
YO-PRO-1 491 506  
YO-PRO-3 613 629  
YOYO-1 491 508, 509  
YOYO-3 612 631