The chemistry of RNA synthesis is identical to the DNA synthesis except for the presence of an additional protecting group at the 2' hydroxyl position of ribose. This position is protected with silyl groups, which are stable throughout the synthesis. The remaining positions on both the sugar and the bases are protected in the same fashion as in DNA.
Bio-Synthesis uses state of the art TOM-chemistry for its RNA synthesis. The use of the 2'-O protective group has several advantages over traditional 2'-O protecting groups such as tBDMS and others. Advantages include the lack of 5'-2' chain elongations, better coupling efficiencies and more. This leads to measurable higher quality of our RNAs compared to our competitors'. Any qustions concerning RNA synthesis chemistry, contact our Technical Support at biosyn@biosyn.com