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What does the DNA OD260nm value stand for ?

The OD260nm (Optical Density) value denotes the amount of oligonucleotide you have received. The conversion of OD260nm values to nanomoles depends on the molecular weight of the individual oligo. Therefore no general applicable conversions can be offered.

1 OD260nm Equals Conversion -  nmole
30 µg ss-DNA 4.5 nmol of a 20mer
40 µg ss-RNA 1.8 nmol of a 50mer
50 µg ds-DNA 0.9 nmol of a 100mer

* only valid for ss-oligos with equal base composition of all four bases.

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