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Endotoxin Limulus Amebocyte Lysate (LAL) Bio-Synthesis Endotoxin Contamination

In order to keep an endotoxin free environment, the following standard operating procedures are implemented:

  1. An ultrapure clean water system is in place; double distilled, reverse osmosis, ion exhange purified water is filtered tru activated carbon and 0.002 micron pore size hollow filters.
  2. Bio-Synthesis, Bio-Active siRNA’s are purified tru HPLC using EDTA containing buffers
  3. All glassware is autoclaved and washed with 0.2 M sodium hydroxide and rinsed with sterile water
  4. Ultra pure water is continuosly checked by external labs for endotoxin contamination
  5. Our threshold values are set to be <than 10 EU/nmol as determined by the Limulus Amebocyte Lysate (LAL) test; also know as the horseshoe crab endotoxin test.

Only a dedicated staff and good SOP’s can assure a reliable RNA product for your in vivo si RNA experioments. We at Bio-Synthesis are committed to provide high purity/quality RNA reagents for your valuable research endeavors.

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