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Peptide synthesis methods

For peptides with less than 100 amino acids, we use chemical method to do the synthesis. For peptides with more than 100 amino acids, we use custom recombinant method. Fmoc solid-phase peptide synthesis is employed to build linear peptides. Peptide is synthesized from its C-terminus by stepwise addition of amino acids. Initially, the first Fmoc-amino acid is attached to an insoluble support resin via an acid labile linker. After de-protection of Fmoc by treatment with piperidine, the second Fmoc-amino acid is coupled utilizing a pre-activated species or in situ activation. After the desired peptide is synthesized, the resin bound peptide is de-protected and detached from the resin via TFA cleavage.

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Greetings, Researchers,

Please be advised that any information, guidelines, write-ups, and oral/video/graphic content on our website are intended solely as general guidelines.

It is the researcher's sole responsibility to conduct thorough research on the design of products intended for their experiments before submitting
their designs to Biosynthesis for production feasibility review.

Thank you for your understanding.

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