Why does the calculated amount of RNA in solution differ from that on the Product Transfer Form

The sample may not be thoroughly mixed. Upon drying, RNA may form aggregates or higher-order structures. To disrupt these, heat samples to 95°C for 1-3 minutes and slowly cool for 30-45 minutes to reanneal complementary strands.

Differences in instrumentation used for quantifying RNA may lead to differences in the apparent values observed. Consider using dual-beam UV-VIS spectrophotometers for accurate measurements.

The sample is too concentrated. Absorbance values are most accurate between 0.15 and 0.6 and within the linear range of a standard curve.

The sample is too diluted. Measurements with dilutions of small volumes (1-1.5 uL) are more susceptible to variation.