Live Chat Support Software
800.227.0627

Development Of A Polyclonal Anti-Sperm Protein 17 Antibody

11/30/2013

Polyclonal antibodies were developed for the detection of Sp17 in human tissues. A recombinant Sp17 protein was expressed and purified for use as an antigen. Polyclonal anti-sera was harvested after seven immunizations with the Sp17 recombinant protein. ELISA analysis detected positive anti-recombinant Sp17 titers as low as 1:500 dilution of the rabbit anti-sera. Furthermore, dot blot analysis revealed that the polyclonal anti-Sp17 sera exhibited high affinity for concentrated Sp17 recombinant protein.