SEC Peptide Desalting

Salt removal, buffer exchange, and polishing steps that improve usability and consistency (GLP / ISO 13485 aligned, non-GMP).

SEC separates primarily by hydrodynamic size and is often used after chromatography for cleanup rather than primary “look-alike” impurity separation.

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When to use What it won’t do Typical workflow Deliverables Related pages

When desalting / SEC is the right choice

Best fit

• Removing salts and small molecules after purification
• Buffer exchange for downstream compatibility
• Polishing to remove large contaminants (case dependent)

Most common placement

• After RP-HPLC or IEX (cleanup step)
• Before lyophilization or formulation steps (as needed)

What SEC typically won’t solve

If your key risk is near-neighbor peptide impurities that are close in size, SEC often lacks the resolution to separate them. In those cases we use RP-HPLC, IEX, HILIC, or orthogonal combinations first, then apply SEC/desalting for cleanup.

Typical workflow

• Primary purification: RP-HPLC / IEX / HILIC (as appropriate)
• Cleanup: desalting or SEC to remove salts/small molecules
• Verify: analytical HPLC + MS identity verification (as scoped)
• Finalize: COA + handling recommendations

Deliverables

• Analytical HPLC chromatograms + purity assessment
• MS identity verification (project-appropriate)
• COA + supporting data package

Non-GMP: GLP / ISO 13485 aligned workflows. GMP manufacturing is not offered.

Related purification pages

RP-HPLC Ion-Exchange (IEX) HILIC COA & QC Purification Overview