Cell line contamination has plagued the scientific community for decades. Cells used in basic and applied research can become altered in culture, either by contamination from other cell lines or by spontaneous genetic changes. The American Type Culture Collection (ATCC) recommends that researchers use
low-passage cell lines and authenticate cell line to assure reliable
and reproducible results. While low passage minimizes the number of spontaneous
changes that may arise, authentication of the cell line provides even more
benefits:
Our standard cell line authentication,
beside being able to detect contaminations and mutations between human cells, can
also verify whether the cell line in question originates from a human by
detecting the absence of standard markers.
Our species-specifics authentication services can selectively verify whether the cell line in question is indeed one of the 14 detectable species using our two-pronged
inter-species identification and detection approach. Intra-species
identification services is currently only available for human cell lines.
(Detectable Species: Pig, Human, Cat, Chinese
hamster, Rhesus monkey, Sheep, Horse, African green monkey, Rat,
Dog, Mouse, Rabbit, Goat, and Bovine)
Cell line authentication can verify whether the genotype of the cell line in question is identical to the genotype of the presumed parental cells. If the parental cells genotypes have been determined by a recognized repository (
ATCC,
DSMZ, or
JCRB), the genotype of the submitted cells can be compared to genotypes in the database. Bio-Synthesis can also genotype any parental cells not already represented in the database.
When should cell line Identification and authentication be conducted?
The National Institutes of Health and increasing numbers of scholarly journals have defined guidelines for the authentication of any cell lines to be used in grant-eligible projects or in papers submitted for publication. Authenticate every time a new line is established or acquired.
Cells should be authenticated again before freezing, once every two months that the culture is actively growing, at any point that the performance of the line is inconsistent or yields unexpected results, and before publication.
Test cells whenever more than one cell line is in active use in the same laboratory to rule out cross-contamination.
Test cells when beginning any new series of experiments.
If you have any questions or would like to inquire about the status of your order, please
contact us.
We accept POs and major credit cards
. The purchase will be listed on your statement as “Bio-Synthesis, Inc.” For international orders, we must apply the full charge at the time the order is placed. In the unlikely event that any given order cannot be filled, we will refund the full amount of the purchase.