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Custom LNA (Lock Nucleic Acid Synthesis), Locked Nucleic Acid (LNA) was first described by Wengel and co-workers in 1998 as a novel class of conformationally restricted oligonucleotide analogues. LNA is a bicyclic nucleic acid where a ribonucleoside is linked between the 2'-oxygen and the 4'-carbon atoms via a methylene unit.
Oligonucleotides containing LNA exhibit unprecedented thermal stability towards complementary DNA and RNA2, which allows excellent mismatch discrimination. In fact, the high binding affinity of LNA oligos is recommended for use in any hybridization assay that requires high specificity and/or reproducibility such as |
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Dual labeled
probes |
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In situ hybridization
probe |
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Allele Specific
PNA |
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mRNA sample preparation |
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| As a result
of these significant characteristics, the use of LNA-modified
oligos in antisense drug development is now coming under
investigation4 and, recently, the therapeutic
potential of LNA has been reviewed.5 |
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| BSI offers LNA synthesis
with great flexibility. For more information on custom LNA
synthesis, call 800-227-0627 or write to
us at biosyn@biosyn.com |
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Deprotected, desalted
and ready to use |
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Multi-scale capacities |
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Purification by PAGE
or RP-HPLC upon request |
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QC analysis by MALDI-TOF
Mass spectrometry, HPLC, PAGE analysis |
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Fast delivery with
high throughput capacity |
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References:
(1a) A.A. Koshkin, S.K. Singh, P. Nielsen, V.K.
Rajwanshi, R. Kumar, M. Meldgaard, C.E. Olsen,
and J. Wengel, Tetrahedron,1998, 54, 3607-3630.
(1b) S.K. Singh, P. Nielsen, A.A. Koshkin, and
J. Wengel, Chem. Comm., 1998, (4), 455-456.
(2) L. Kværnø and J. Wengel, Chem. Comm., 1999,
(7), 657-658.
(3) P. Mouritzen, A.T. Nielsen, H.M. Pfundheller,
Y. Choleva, L. Kongsbak, and S. Møller, Expert
Review of Molecular Diagnostics, 2003, 3(1),
27-38.
(4a) J. Kurreck, E. Wyszko, C. Gillen, and V.A.
Erdmann, Nucleic Acids Res., 2002, 30, 1911-1918.
(4b)H. Ørum and J. Wengel, Curr. Opinion in
Mol. Therap., 2001, 3, 239-243.
(5a) M. Petersen and J. Wengel, Trends in Biotechnology,
2003, 21(2), 74-81.
(5b) D.A. Braasch, D.R. Corey, Biochemistry,
2002, 41, 4503-4510. |
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| The most trusted name in Quality
Custom Synthesis. |
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