40+ years of excellence in custom synthesis and bioconjugation services.
Calculators, design tools, and educational content to support your research.
Precision RNA synthesis for conventional guides, engineered scaffolds and chemically modified CRISPR RNA designs. Built around your sequence, application and analytical requirements.
Length, modifications, yield, purity and delivery format are evaluated for each project.
Guide RNA sequence, structure and chemistry influence nuclease recognition, intracellular persistence and experimental performance. Bio-Synthesis provides custom RNA synthesis for researchers developing conventional guides, modified constructs and longer RNA designs that require individualized feasibility assessment.
Up to 400 nt refers to the available custom RNA synthesis length capability—not a guarantee that every 400-nt sequence, modification pattern or purity target can be achieved with the same process.
Need RNA longer than 400 nt? For longer RNA transcripts, explore our Custom RNA Transcription Services (in vitro transcription, IVT). Our team can help evaluate the appropriate production approach for your sequence, length and application.
Order sequence-specific sgRNA, crRNA or tracrRNA designs compatible with your selected nuclease system.
Evaluate terminal or internal chemical modifications to address stability, handling or experimental objectives.
Discuss longer guide-associated RNA architectures, including specialized scaffold and engineered designs, up to 400 nt.
These illustrative, color-coded RNA sequences are not actual guide designs. Exact lengths and structural elements vary by CRISPR system and nuclease.
A single RNA construct combines a target-recognition region with a nuclease-binding scaffold. Commonly used in compatible Cas9 editing workflows.
Illustrative RNA bases (A, U, G, C) · colors distinguish nucleotides · not a validated guide sequence
Typical guide lengths vary with the Cas protein and engineered scaffold.
Modification patterns should be selected with the intended Cas protein, RNA architecture and delivery method in mind. Not every modification is suitable at every nucleotide position.
Design note: Modification strategies reported for one Cas9 guide are not automatically transferable to Cas12, prime-editing guide RNAs or other systems. Functional validation remains application-specific.e choice of capping strategy. Chemical capping of longer RNAs may also present purification challenges.
Different editing platforms use distinct guide architectures. Our team can review the requested RNA sequence, length and chemistry against the intended application.
sgRNA or compatible crRNA:tracrRNA formats for sequence-directed DNA editing research.
crRNA designs tailored to the selected Cas12 nuclease and its guide requirements.
Guide RNAs for nuclease-inactive CRISPR platforms used to modulate gene expression.
Guide RNA synthesis for compatible base-editor constructs and experimental workflows.
Discuss longer prime-editing guide RNA (pegRNA) architectures and extension requirements.
Guide RNA supply for in vitro ribonucleoprotein assembly and subsequent research assays.
Our custom synthesis process starts with a technical review rather than assuming a single workflow fits every guide RNA.
Share sequence, format, Cas system, modifications and intended use.
Assess RNA length, synthesis strategy, purification and requested yield.
Produce the custom RNA and apply agreed analytical methods.
Provide project-specific results and delivery specifications.
Purity, identity and integrity are particularly important for longer and chemically modified RNA constructs. Analytical methods and release specifications should be agreed upon before synthesis.
Bio-Synthesis operates quality systems supporting custom research materials. Any GMP, clinical-use, endotoxin or special release requirements must be discussed and confirmed separately.
Specific analytical methods, purity thresholds and achievable yields are quotation-dependent.
Explore complementary RNA synthesis and modification capabilities for CRISPR guide development, longer RNA constructs, and RNA research workflows.
Share your guide format, sequence, modifications, synthesis scale and QC needs for a project-specific review.
sgRNA · crRNA · tracrRNA
5′→3′ RNA; up to 400 nt
2′-OMe · 2′-F · PS
Cas9 · Cas12 · extended guide
Target yield & number of guides
Purity · identity · CoA
Single-guide, dual-RNA and extended guide designs tailored to the CRISPR platform.
Sequence-dependent modification planning, purification, identity confirmation and documentation.
These publications provide background on guide RNA engineering and chemical modification; they do not imply that Bio-Synthesis manufactured the materials used in these studies.
Selected peer-reviewed background on CRISPR guide RNA engineering, chemical stabilization and extended guide designs.
These references are for scientific context and do not indicate that Bio-Synthesis supplied materials used in the studies. Guide RNA performance depends on sequence, Cas system and experimental conditions.
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