Long Synthetic Custom RNA Oligos
While there has been significant demand for solid phase synthesis of long RNA
fragments such as miRNA, shRNA in gene structure and functional studies, the
ability to synthesize longer oligos is highly sequence-dependent due to the
potential for secondary structure formation and the inherent difficulty for
chemical synthesis with additional 2'-OH reactive group of RNA introduces
considerable complexity to the synthesis. Therefore, an effective chemical
synthetic method for longer RNA is an important basic technology for further
advancement on RNA related research.
Bio-Synhthesis has met the challenge of synthesizing thousands of synthetic long
RNA, RNA UbermerTM up to 200 bases and d2RNATM Long Oligos (3000 bases and more)
using the best reagents available, proprietary methods to optimized synthesis
protocols and state-of-art synthesis facilities and expertise in providing
oligonucleotide synthesis services since 1984.
Each RNA oligo is meticulously monitored during synthesis and controlled according
to Bio-Synthesis’s stringent quality assurance and quality control standards. The
final product is identified by LC-MS Electrospray, sequencing or other custom analytical
methods. Purity is analyzed by high throughput capillary gel or PAGE electrophoresis.
For over 25 years Bio-Synthesis has maintained these gold standards for long RNA
oligonucleotide synthesis that saves the researcher a great deal of time and trouble
for applications that require direct synthesis of the entire target fragment such
as mRNA.
Applications
These long oligos can be used in variety of molecular biology application including
but not limited to:
- RNA control template for real-time PCR
- mRNA for gene expression
- Quantitative control for qPCR
- Internal controls for house-keeping genes
- External RNA spike-in control assays
- In-situ Hybridization RNA probes
- Expression control via anti-sense RNA
- RNA template for in vitro translation
Purification
All Bio-Synthesis oligos include desalting and deprotection at no additional
charge. We strongly recommend oligonucleotide longer than 50 bases to be
purified by RNase free poly acrylamide gel electrophoresis (PAGE). If you are
unsure about the purification level you need for your research, see our
technical bulletin titled What type of purification I need for my applications or
contact us for assistance.
Pricing
Unlike other suppliers, there is no additional charge for RNA Oligo UbermerTM Synthesis.
Minimum Yield
Yield will vary based upon base composition, purification, oligo length, scale and
modifications. That's why we have a set guaranteed quantity.
Guaranteed Quality
Oligos are routinely checked for length, purity, and composition. Long oligos are
typically quality checked by PAGE, ESI-MS, and/or CE. Our global quality control
capabilities include MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry
(ESI-MS), capillary electrophoresis (CE), and polyacrylamide gel electrophoresis
(PAGE). If you are dissatisfied and report an oligo problem within 30 days of order
receipt, we will gladly remake your oligo free of charge or provide a credit for
the purchase amount.
All orders are shipped with a Quality Assurance document that includes measured
yields, melting temperature, MW and µg/OD with every oligo.