Enhanced Diagnostic Tools
DyLight Fluors are a complete family of high-intensity, photostable fluorescent dyes for labeling antibodies and other molecular probes for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms. All ten DyLight Fluorescent Dyes are offered in both NHS-ester-activated and maleimide-activated forms, providing for efficient and specific labeling of primary amines and sulfhydryl groups, respectively. (See Related Products below for other DyLight Products, including antibody labeling kits, conjugated secondary antibodies and streptavidin, and molecular weight markers.)
DyLight Fluorescent Dyes have absorption spectra ranging from 353 nm to 777 nm (Table 1) and match the principal output wavelengths of common fluorescence instrumentation. The DyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa Fluor*, CyDyes* and LI-COR* dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio without precipitation during conjugation.
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