Sequence Detection Systems Quantitative Assay Design and Optimization

Introduction

PE Biosystems has developed guidelines enabling streamlined design and implementation of real-time quantitative PCR assays. The use of these guidelines makes it easy to apply either the fluorogenic 5´ nuclease assay or SYBR® Green I double-stranded DNA binding dye chemistry to any real-time quantitative PCR system. Specific assay design and optimization guidelines minimize the time and cost of assay implementation, while providing reliable and robust assay performance.