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FLAG Tag Peptide

FLAG Tag Peptide
Sequence (1LC):
DYKDDDDK
Sequence (3LC):
H - Asp - Tyr - Lys - Asp - Asp - Asp - Asp - Lys - OH
Catalog :
13505-01
Unit:
5 mg
Price/Unit:
$642  (For other quantities please Contact Us or call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442)
Purity:
>95%
Format:
Lyophilized solid, trifluoroacetate (TFA) salt format
Description:
Bio-Synthesis provide FLAG peptide on demand or custom large scale FLAG peptide synthesis. This tag peptide consist of eight amino acids Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys as part of enterokinase-cleavage site, was widedly used for protein identification and immunoaffinity chromatography. This synthetic peptide can be elute under non-denaturing conditions. Several of antipeptide antibody have been developed example such as M1 can be used to binds the peptide in the presence of bivalent metal cations, preferably Ca+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAG peptide.
Storage:
-20°C
Product Usage:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
M.W.:
1013
Reference/Citations:
References:
  1. A. Einhauer, A. Jungbauer. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49 2001 455–465.
  2. Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A 1981;78:3824–8.
  3. Hopp TP. Protein surface analysis: methods for identifying antigenic determinants and other interaction sites. J Immunol Methods 1986;88:1–18.
  4. Power BE, Ivancic N, Harley VR, Webster RG, Kortt AA, Irving RA, et al. High-level temperature-induced synthesis of an antibody VH-domain in Escherichia coli using the pel B secretion signal. Gene 1992;113:95–