Hemin-modified oligonucleotides are soluble in water; hence ultrapure water is the best solvent for reconstituting hemin-modified oligonucleotides.
A note of caution: Tris-EDTA (TE) buffer might not be a good solvent since EDTA in the TE buffer might destroy Hemin by chelating iron (Fe).
Please note: G4 DNA-hemin conjugates are useful DNAzymes for sensitive detection in colorimetric biosensor applications. Hemin also binds to human serum albumin in plasma.
References
Hemin
Kosman J, Żukowski K, Juskowiak B. Comparison of Characteristics and DNAzyme Activity of G4⁻Hemin Conjugates Obtained via Two Hemin Attachment Methods. Molecules. 2018 Jun 9;23(6):1400. [PMC]
Udomprasert A, Chimasungkanun S, Kangsamaksin T. Kinetic analysis of catalytic activity of G-quadruplex/hemin DNAzyme with flanking adenine nucleotides. Sci Rep. 2025 Nov 17;15(1):40260. [PMC]
Zunszain PA, Ghuman J, Komatsu T, Tsuchida E, Curry S. Crystal structural analysis of human serum albumin complexed with hemin and fatty acid. BMC Struct Biol. 2003 Jul 7;3:6. [PMC]