Black Hole Quencher 2 - BHQ2 Oligonucleotide Modification

Bio-Synthesis offers Black Hole Quencher®-1 dye oligonucleotide modification. BHQ-2, a non-fluorescent dye can be place at 5' or 3' terminus or internally of an oligonucleotide. BHQ®-2 labeled oligonucleotides are used in many applications, including dual-labeled fluorogenic probes for real-time PCR. This dark quencher has strong absorption range of 599-670  nm, which provides excellent quenching of fluorophores that fluoresce in this range, such as Quasar® 570, TAMRA, CAL Fluor® Red 590, CAL Fluor Red 610, ROX, CAL Fluor Red 635, Pulsar® 650, Quasar 670 and Quasar 705 dyes. BHQ-2 dye may quench fluorescence by both FRET and static quenching mechanisms. Contact Bio-Synthesis for BHQ2 Quencher Oligonucleotide Synthesis and Modification Services.

Product Information

 

Product Name:

Black Hole Quencher 2 - BHQ2 Oligonucleotide Modification

Alternate Name:

BHQ2

Category:

Quncher

Modification Code:

[BHQ2]

Chemical Formula:

C24H25N6O8P

Exact Mass:

556.15

Formula Weight:

556.47

m/z:

556.15 (100.0%), 557.15 (26.6%), 558.15 (5.5%), 557.14 (2.2%)

Elemental Analysis:

C, 51.80; H, 4.53; N, 15.10; O, 23.00; P, 5.57

Absorption λmax:

579 nm

Structure:

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

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