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BODIPY TR dye can be incorporated at any position within an Oligonucleotide post-synthetically. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS ester chemistry; other cross-linking chemistries are also available depending on particular project specifications . Dual HPLC purification is highly recommended.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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