DBCO PEG8 NHS
DBCO-PEG8 NHS ester is a versatile bioconjugation reagent used to prepare DBCO-functionalized oligonucleotides through reaction with amino-modified DNA, RNA, PNA, aptamers, probes, antisense oligonucleotides, and other nucleic acids. The NHS ester reacts efficiently with primary amines, while the DBCO group remains available for subsequent strain-promoted azide-alkyne cycloaddition (SPAAC) with azide-containing molecules.
Compared with standard DBCO NHS ester, the PEG8 linker provides a longer and more hydrophilic spacer, improving aqueous solubility and reducing steric interference during conjugation. This often enhances reaction efficiency when coupling larger biomolecules such as antibodies, enzymes, proteins, nanoparticles, or polymers.
The DBCO moiety reacts rapidly and selectively with azides under physiological conditions without requiring copper catalysts, making it ideal for copper-free click chemistry involving sensitive biological systems.
Typical applications include:
- Copper-free click chemistry (SPAAC)
- Antibody–oligonucleotide conjugates
- Peptide–oligonucleotide conjugates
- Protein labeling
- Nanoparticle functionalization
- PEGylation
- Surface immobilization
- Biosensor development
- DNA nanotechnology
- Targeted delivery research
- Fluorescent probe preparation
Bio-Synthesis offers custom DBCO-PEG8-modified oligonucleotides prepared by conjugating amino-modified oligonucleotides with DBCO-PEG8 NHS ester for high-efficiency bioorthogonal conjugation.
Technical note: Like DBCO NHS ester, DBCO-PEG8 NHS ester is a post-synthetic labeling reagent, not a phosphoramidite. It is typically reacted with a 5′-, 3′-, or internally amino-modified oligonucleotide (such as Amino C6 or Amino-TEG) to generate the final DBCO-PEG8-modified construct. The PEG8 spacer generally offers improved solubility and greater flexibility than shorter PEG linkers such as PEG2 or PEG4.
Typical Specifications
| Property |
Specification |
| Modification |
DBCO-PEG8 |
| Reagent |
DBCO-PEG8 NHS Ester |
| Reactive Groups |
DBCO (cyclooctyne) and NHS ester |
| Spacer |
Polyethylene glycol (PEG8) |
| Conjugation Chemistry |
SPAAC (Copper-free Click Chemistry) |
| Copper Requirement |
None |
| Reaction Partner |
Azide-modified molecules |
| Typical Placement |
5′ end, 3′ end, or internal amino-modified positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, probes |
| Typical Applications |
Copper-free click chemistry, antibody conjugation, peptide conjugation, protein labeling, nanoparticle functionalization, biosensors, targeted delivery |
Product Information
copper-free click chemistry
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Agard NJ, Prescher JA, Bertozzi CR. A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. J Am Chem Soc. 2004;126(46):15046-15047.
- Jewett JC, Bertozzi CR. Cu-free click cycloaddition reactions in chemical biology. Chem Soc Rev. 2010;39:1272-1279.
- Sletten EM, Bertozzi CR. Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angew Chem Int Ed. 2009;48:6974-6998.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
No Product Found