Alanine scanning is a systematic method used to determine which amino acids within a peptide are critical for binding or biological activity.
In this approach, each residue in a parent peptide is individually replaced with alanine (or another neutral residue). The modified peptides are then tested to evaluate changes in activity.
Loss or reduction of activity after substitution indicates that the original residue plays an important role in binding.
To automatically generate alanine substitution panels, use our Peptide Screening Tools:
https://www.biosyn.com/peptidescreeningtools.aspx
The software allows you to input a sequence and automatically create a complete alanine scan panel ready for export and synthesis.
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