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Mass Changes - protein sequencing and analysis

Mass Change
Modification Monoisotopic Mass Change Average Mass Change
Homoserine formed from Met by CNBr treatment -29.9928 -30.0935
Pyroglutamic acid formed from Gln -17.0265 -17.0306
Disulphide bond formation -2.0157 -2.0159
C-terminal amide formed from Gly -0.9840 -0.9847
Deamidation of Asn and Gln 0.9840 0.9847
Methylation 14.0157 14.0269
Hydroxylation 15.9949 15.9994
Oxidation of Met 15.9949 15.9994
Proteolysis of a single peptide bond 18.0106 18.0153
Formylation 27.9949 28.0104
Acetylation 42.0106 42.0373
Carboxylation of Asp and Glu 43.9898 44.0098
Phosphorylation 79.9663 79.9799
Sulphation 79.9568 80.0642
Cysteinylation 119.0041 119.1442
Pentoses (Ara, Rib, Xyl) 132.0423 132.1161
Deoxyhexoses (Fuc, Rha) 146.0579 146.1430
Hexosamines (GalN, GlcN) 161.0688 161.1577
Hexoses (Fru, Gal, Glc, Man) 162.0528 162.1424
Lipoic acid (amide bond to lysine) 188.0330 188.3147
N-acetylhexosamines (GalNAc, GlcNAc) 203.0794 203.1950
Farnesylation 204.1878 204.3556
Myristoylation 210.1984 210.3598
Biotinylation (amide bond to lysine) 226.0776 226.2994
Pyridoxal phosphate (Schiff Base formed to lysine) 231.0297 231.1449
Palmitoylation 238.2297 238.4136
Stearoylation 266.2610 266.4674
Geranylation 272.2504 272.4741
N-acetylneuraminic acid (Sialic acid, NeuAc, NANA, SA) 291.0954 291.2579
Glutathionylation 305.0682 305.3117
N-glycolylneuraminic acid (NeuGc) 307.0903 307.2573
5’-Adenosylation 329.0525 329.2091
4’-Phosphopantetheine 339.0780 339.3294
ADP-ribosylation (from NAD) 541.0611 541.3052