5' capped oligos

• 5' Triphosphate cap (5'-Triphosphate RNA, 5'-PPP RNA)
• Guanosine-triphosphate Cap (5' Gppp)
• 5' N7-methylguanosine-triphosphate Cap (5' N7-MeGppp, 7mGppp, mRNA Cap)
• 5' Adenylated (rApp, 5' Adenylation)

Capped RNA oligonucleotides obtained by chemical synthesis have several advantage over capped RNAs generated by in vitro transcription. 5' capped oligos via chemical synthesis can be:

• Scalable, robust and reproducible
• Modified chemically at 5' position
• Deliver purified
• QC by mass spectromettry, anion-exchange HPLC, reverse phase HPLC and PAGE

• Triphosphate modified RNA, as antivirus agent by stimulate the immunoreceptor RIG-I and protect mice from influenza in vivo.
• RNA triphosphates are used in the synthesis of long RNA oligos using RNA ligation.
• RNA oligos with a triphosphate group at the 5' end induce anti-viral immunity via TLR-independent pathways.
• 5' triphosphate RNA are important in the enzymatic synthesis of m7G-5'-capped RNAs.
• This process protects the mRNA and is important for translation.
• Triphosphate modified DNA can be used as a substrate for the preparation of synthetic genes.

Gunosine-triphosphate Cap (5' Gppp)