7-Deaza-8-aza-dA DNA Modification

 7-deaza-8-aza-dA, and 7-deaza-8-aza-A lack group that are critical for hydrogen bonding.  7-Deaza-8-aza-A and 7-deaza-8-aza-G (PPG) monomers are isomers of A and G and have similar electron density. Their presence in oligos is slightly stabilizing relative to A and G.

7-deaza modified base does not lead to aggregation in G-rich oligos and can be easily prepared and isolated. 5' fluorescein labeled oligos with 7-deaza-8-aza A modified base are much less quenched than the equivalent G oligos.

Product Information

 

Product Name:

7-Deaza-8-aza-dA DNA Modification

Category:

Modified Bases

Modification Code:

[7-Deaza-8-Aza-dA]

Chemical Formula:

C10H12N5O5P

Exact Mass:

313.06

Formula Weight:

313.21

Structure:

Purification:

HPLC or PAGE

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Related Products:

3-Deaza-5-Aza-2'-O-methylcytidine Oligonucleotide Modification

7-Deaza-2'-deoxyadenosine, 7-Deaza-dA

7-deaza-2'-deoxyguanosine, 7-Deaza-dG

7-Deaza-2'-deoxyxanthosine, 7-Deaza-dX

7-Deaza-Adenosine, 7-Deaza-A RNA Modification

7-deaza-8-aza-dG DNA Modification

3-deaza-2'-deoxyadenosine, 3-deaza-dA

References/Citations:

1.  F. Seela and H. Driller, Helvetica Chimica Acta, 1988, 71, 1191-1198.
2.  F. Seela and K. Kaiser, Helvetica Chimica Acta, 1988, 71, 1813-1823.
3.  F. Seela and G. Becher, Nucleic Acids Res., 2001, 29, 2069-2078.
4.  L.V. Nechev, I. Kozekov, C.M. Harris, and T.M. Harris, Chem Res Toxicol, 2001, 14, 1506-1512.

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