Template Submission Guide for mRNA Transcription.

This guide outlines requirements for submitting plasmid DNA or PCR templates for mRNA production workflows. Plasmid DNA Requirements

• T7 promoter (preferred) upstream of sequence
• Unique restriction site at 3′ end
• High purity DNA

PCR Template Requirements

• Correct promoter sequence included
• High-quality amplification
• Minimal impurities

DNA Quantity Guidelines

• ~5 µg plasmid recommended
• Extra DNA advised due to linearization loss

Important Considerations

• Proper sequence orientation
• Promoter placement accuracy
• Sequence verification recommended

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