Enhanced Diagnostic Tools
Bio-Synthesis has developed sensitive analytical methods for many types oft est agents including small molecules, peptides, DNA RNA and proteins. Each of these used in many different types of biological matrices such as plasma, brain, urine, liver, heart, and fat.
Complete bioconjugation based drug discovery and development program for pharmaceutical and biotech companies.
AABB Accredited DNA testing laboratory for DNA identity and relationship analysis.
High resolution and low resolution HLA typing by using Luminex SSO and PGR- SSP typing system.
Over 6,000 catalog peptides ready to use. All > 95% purity.
30,000-1- high quality monoclonal and polyclonal antibodies from different host species applicable to all areas of biological research and drug discovery.
5’-d(TTTTTmCTTTmCTmCTmCT)-3’; T = BNANC[NH]
5’-d(GCTAAAAA GAAA GA GA GATCG)-3’
3’-d(CGATTTTT CTTT CT CT CTAGC)-5’ Target dsDNA
1D, 2D, native, reduced
Peptides, Oligonucleotides, Carbohydrates,Conjugates, etc.
Peptide, Proteins, Oligonucleotides,Carbohydrates, Lipids, etc.: Various LC methods
Sequencing (N-terminal, internal, C-terminal)
Peptides, Oligonucleotides, Conjugates, etc.
Peptide, Proteins, Oligonucleotides, Metabolites Various LC methods may be used
Transfer sandwich from cathode (-) to anode (+)
4-12 % Bis-Tris Gradient Gel
50 mM MES-Tris, 3.465 mM SDS, 1.025 mM EDTA pH 7.3, 180 to 200 V,~ 1.5 h.
1 x NuPage transfer buffer (25 mM Bis-Tris, 25 mM Bicine, 1 mM EDTA, pH 7.2) + 20 % methanol, 1 h at 18 mA, Immobilon-P (PVDF membrane from Millipore),~ 1 h.
15 to 30 Minutes in 50% Methanol, 10% acetic acid + comassie blue R250 (1 g/L)
10 Minutes to hours in 50% Methanol, 10% acetic acid.
From Laursen et al. 2005 Microbiol. Mol Biol Rev. 69:101
Garcia C, Fortier PL, Blanquet S, Lallemand JY, Dardel F
Solution structure of the ribosome-binding domain of E. coli translation initiation factor IF3. Homology with the U1A protein of the eukaryotic spliceosome J. Mol. Biol. v254, p.247-259
Initiation of translation in prokaryotes requires the formation of a complex between the messenger RNA, the 30 S ribosomal subunit and the initiator tRNA(fMet). Initiation factor IF3 binds to the 30 S ribosomal subunit and proof-reads the initiation complex, thereby ensuring the accuracy of this step. IF3 also plays a pleiotropic role in the regulation of translation, as a result of differential influences exerted on the levels of the initiation of translation of genes or groups of genes....
Gel bands labeled cut out and reconstituted over night in 500 ml extraction buffer (5% formic acid in 50% acetonitrile). Cellulose paper was removed. The gel bands were digested using a modified method similar to the one used by Shevchenko et al., 1996. LC-MS and LC-MS/MS analysis was performed using a Microm-LC and an LCQ deca.
Mass spectrum at retention time 32.2 (lower trace, 5639). Note: A peak with a mass envelope from 1353 to 1381 was detected. The presence of these peak indicates that the KAEEAGVDLVEISPNAEPPVCR is labeled with the AMS fluorophor at the cysteine.
FT MOD_RES 1
FT MOD_RES 3
Symmetric dimethylarginine (alternate) (By similarity)
FT MOD_RES 5
N6-acetyllysine (By similarity)
FT MOD_RES 8
FT MOD_RES 12
FT MOD_RES 16
FT MOD_RES 20
Purification of H4 (lane3) from a commercially available histone preparation (lane 2) by ion exchange and reversed-phase chromatography
Mass Range: 575-1300 m/z ; MaxEnt: 10-13 Kd; Zoom: 11.3-11.52 Kd
James R. Walker,1* Annie J. Gnanam,1 Alexandra L. Bllnkova,1 Mary Jo Hermandson, 1F Mikhail A. Karymov,3 Yuri L. Lyubchenko,2 Paul R. Graves,38 Timothy A. Haysteac3 and Klaus D. Linse4
1 Molecular Genetics and Microbiology Section and institute for Cellular and Molecular Biology, University of Texas Austin, TX 78712. USA
2Department of Pharmaceutical Sciences, College of Pharmacy University of Nebraska Medical Center. Omaha, NE 68198. USA
3Department of Pharmacology, Duke University Medical Center, Durham, NC27710, USA.
4Protein Analysis Facility institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.
Endospores formed by members of the Clostridium/ Bacillus subphylum typically consist of a dehydrated core containing the chromosome, a double membrane layer, a peptidoglycan cortex, and a proteinaceous coat - struc-tures which allow spore survival of extremely harsh conditions (Driks. 2003). The principal core proteins -small acid-soluble proteins - bind and saturate the DNA (Setlow. 1966; Driks. 2002a.b). The cortex is a specialized peptidoglycan. which constricts and maintains dehydra¬tion within the core (Popham etaf.: 1995; Driks: 2003).
Present in P29a
a. X represents an unknown residue; (GVT) could be G or T
b The PES internal peptides. 4, 5 and 14 were initially sequenced as YSLSLTTLSLP, VFFGLESS and SYSVNLTNG but corrected upon re-esannination of the raw data in light of the deduced amino acid sequences of the P£9 genes. Mess spectrometry could not distinguish I and L; the final assignments were based on the deduced amino acid sequences.
Fairman`& Akerfeldt, 2005
Multiply charged ions of the analyte (e.g., a protein sample) are formed by protonation in an acidic solvent One, several, or many positive charges are detected Analyte is sprayed through a high-voltage nozzle into a vacuum chamber Solvent evaporate, leaving ‘naked’ ions of the multiply charged analyte Ions are detected according to mass-to-charge (m/z) ratio Detected ions are displayed as a series according to m/z ratio Computer deconvolution of the m/z peak series leads to the molecular weight of the analyte
Search m/z Mass Tolerance (Da) # Hits Database
Roepstorff, P and Fohlman, J, Proposal for a common nomenclature for sequence ions in mass spectra of peptides. Biomed Mass Spectrom, 11(11) 601 (1984).
* As reported in UniProt. Potential PTMs have not been verified by Sigma.
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