Synthetic peptides for antibody discrimination. Synthetic peptides can be used for serotype discrimination.
Routsias et al. in 2014 developed a novel ELISA capable of discriminating antibodies targeting different enterovirus serotypes to allow for the characterization of host immune response against different enteroviral serotypes by distinguishing IgM and IgG reactivity and to monitor IgG seroconversion in serial samples.
The researchers employed ELISA for the examination of peptide recognition specificity by IgM antibodies. Synthetic peptides selected for this study were derived from a non-conserved region of VP1 protein of coxsackieviruses B2, B3, B4, B5, A9, A16, A24, echoviruses 6, 7, 9, 11, 30, enterovirus 71 and parechovirus 1. Using this approach the scientists demonstrated that this assay can be used to discriminate between antibodies targeting different enterovirus serotypes.
Routsias JG, Mavrouli MD, Antonaki G, Spanakis N, Tsakris A.; Synthetic peptides for efficient discrimination of anti-enterovirus antibodies at the serotype level. Peptides. 2014 Aug;58:52-9. doi: 10.1016/j.peptides.2014.04.017. Epub 2014 Jun 11. PMID: 24929043
http://routsias-lab.gr/Synthetic peptides for efficient discrimination of anti-enterovirus antibodies at the serotype level.pdf